Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
All lanes : Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/ml
Lane 1 :
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 57 kDa
Additional bands at : 30 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
The band observed at 53 kDa could potentially be a cleaved form of BMPR1B due to presence of a 13 amino acid signal peptide.
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