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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-BMPR2 antibody
See all BMPR2 products (5) ...
Description
Goat polyclonal to BMPR2
Specificity
By immunoblotting, the antibody shows approximately 10% cross-reactivity with recombinant mouse BMPRIB using reducing conditions, and shows <1% cross-reactivity with recombinant human BMPRIA and recombinant mouse BMPRIA.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human) extracellular.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.% PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Bone Morphogenic Proteins (BMP) are members of the TGF beta superfamily that affect bone and cartilage formation. Mature BMPs are 30-38 kDa proteins that assume a TGF beta-like cysteine knot configuration. Unlike TGF beta, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported. Recently it was found that Lovostatin widely used for lowering cholesterol, also increases bone formation by turning on a gene (bmp2) that promotes local bone formation. BMPs create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis. BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway. There are six TGF beta family type I receptors and four TGF betaƒnfamily type II receptors. BMP receptors are a family of serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B (50-55 kDa) and the type II receptor BMPR2 (70-80 kDa). These receptors are also closely related to the activin receptors ACVR1 and ACVR2.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Signaling Pathways >> TGF beta >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect a limit of ~2.5 ng/well of recombinant human BMPR2.
WB: Use at a concentration of 0.1 - 0.2 µg/ml (determined using human BMPR2 at 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively). Predicted molecular weight: 70-80 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs.
Tissue specificity
Highly expressed in heart and liver.
Involvement in disease
Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1) [MIM:178600]. PPH1 is a rare autosomal dominant disorder characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death. The disease can occur from infancy throughout life and it has a mean age at onset of 36 years. Penetrance is reduced. Although familial PPH1 is rare, cases secondary to known etiologies are more common and include those associated with the appetite-suppressant drugs.
Defects in BMPR2 are a cause of pulmonary venoocclusive disease (PVOD) [MIM:265450]. PVOD is a rare form of pulmonary hypertension in which the vascular changes originate in the small pulmonary veins and venules. The pathogenesis is unknown and any link with PPH1 has been speculative. The finding of PVOD associated with a BMPR2 mutation reveals a possible pathogenetic connection with PPH1.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ13873
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BMP type II receptor antibody
- BMP type-2 receptor antibody
- BMPR 2 antibody
see all
Database links
- Entrez Gene: 659 Human
- Entrez Gene: 12168 Mouse
- Omim: 600799 Human
- SwissProt: Q13873 Human
- SwissProt: O35607 Mouse
- Unigene: 471119 Human
- Unigene: 391654 Mouse
- Unigene: 7106 Mouse
References for Anti-BMPR2 antibody (ab10862)
This product has been referenced in:
- Carroll Met al. Null mutation of the transcription factor inhibitor of DNA binding 3 (id3) affects spermatozoal motility parameters and epididymal gene expression in mice. Biol Reprod 84:765-74 (2011). WB.Read more (PubMed: 21148110) »
- Hamid Ret al. Penetrance of pulmonary arterial hypertension is modulated by the expression of normal BMPR2 allele. Hum Mutat 30:649-54 (2009). WB; Human.Read more (PubMed: 19206171) »
See all 2 publications for this product
Publishing research using ab10862? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"