BMPR2 antibody (ab10862)

Product Name 

BMPR2 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to BMPR2

Immunogen 

Recombinant full length protein (Human) extracellular.

Reacts with 
(species key)

Hu, Ms

Specificity 

By immunoblotting, the antibody shows approximately 10% cross-reactivity with recombinant mouse BMPRIB using reducing conditions, and shows <1% cross-reactivity with recombinant human BMPRIA and recombinant mouse BMPRIA.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect a limit of ~2.5 ng/well of recombinant human BMPR2.
WB: Use at a concentration of 0.1 - 0.2 µg/ml (determined using human BMPR2 at 50 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively). Predicted molecular weight: 70-80 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; Single-pass type I membrane protein.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Signaling Pathways >> TGF beta >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Signal Transduction >> Growth Factors/Hormones >> TGF
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

The bone morphogenetic protein (BMP) receptors belong to a family of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. Both activins and TGF-beta transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors. BMPRs play a crucial role during development as mutations or deletions to the BMPR genes can cause juvenile polyposis, disrupt normal dorsal/ventral patterning during limb development, and may be a factor in the progession of Cowden-like syndrome. Germline mutations in the BMPR2 gene encoding bone morphogenetic protein (BMP) type II receptor (BMPR-II) have been reported in patients with primary pulmonary hypertension (PPH).

Bone Morphogenic Proteins (BMP) are members of the TGF beta superfamily that affect bone and cartilage formation. Mature BMPs are 30-38 kDa proteins that assume a TGF beta-like cysteine knot configuration. Unlike TGF beta, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported. Recently it was found that Lovostatin widely used for lowering cholesterol, also increases bone formation by turning on a gene (bmp2) that promotes local bone formation. BMPs create an environment conducive for bone marrow development by stimulating the
production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis. BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway. There are six TGF beta family type I receptors and four TGF betaƒnfamily type II receptors. BMP receptors are a family of serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B (50-55 kDa) and the type II receptor BMPR2 (70-80 kDa). These receptors are also closely related to the activin receptors ACVR1 and ACVR2.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 0.% PBS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for BMPR2 antibody (ab10862)

  This product has been used in:

Qin W et al. BMPRII is a direct target of miR-21. Acta Biochim Biophys Sin (Shanghai) 41:618-23 (2009). WB; Human. PubMed: 19578724

If you publish research using ab10862 please let us know so that we can cite the reference on this datasheet.

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