![Western blot - BMX antibody [Y396] (ab32153) image](#Wb BMX.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-BMX antibody [Y396]
See all BMX products (8) ...
Description
Rabbit monoclonal [Y396] to BMX
Specificity
Ab32153 recognises BMX.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the pleckstrin homology domain of human BMX.
Positive control
Hela cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide.
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4.
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
Y396
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Western blot - BMX antibody [Y396] (ab32153)
![Western blot - BMX antibody [Y396] (ab32153) image](/ps/datasheet/Images/32/ab32153/Wb BMX.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: 1/50.
WB: 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 78 kDa).
Is unsuitable for FACS or ICC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
Tissue specificity
Preferentially expressed in epithelial and endothelial cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
SH2 domain mediates interaction with RUFY1.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP51813
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bone marrow tyrosine kinase gene in chromosome X protein antibody
- Bmx antibody
- BMX non receptor tyrosine kinase antibody
see all
Database links
- Entrez Gene: 660 Human
- Entrez Gene: 12169 Mouse
- Entrez Gene: 367786 Rat
- Omim: 300101 Human
- SwissProt: P51813 Human
- SwissProt: P97504 Mouse
- Unigene: 495731 Human
- Unigene: 504 Mouse
Anti-BMX antibody [Y396] images:
Western blot - BMX antibody [Y396] (ab32153)
Anti-BMX antibody [Y396] (ab32153) at 1/500 dilution + HeLa cell lysate
Predicted band size : 78 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
References for Anti-BMX antibody [Y396] (ab32153)
ab32153 has not yet been referenced specifically in any publications.
Publishing research using ab32153? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"