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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab59360? Please let us know so that we can cite the reference in this datasheet
ab59360 has been referenced in 1 publications.
- Guryanova OA et al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF; Mouse. PubMed: 21481791
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - BMX antibody (ab59360)
All lanes : Anti-BMX antibody (ab59360) at 1/500 dilution
Lane 1 : Extracts from A549 cells with no immunizing peptide
Lane 2 : Extracts from A549 cells with immunizing peptide
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - BMX antibody (ab59360)
ab59360, at a 1/50 dilution, staining human BMX in thyroid gland, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
Immunocytochemistry/ Immunofluorescence-BMX antibody(ab59360)
ICC/IF image of ab59360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59360, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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