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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-BMX antibody
See all BMX products (8) ...
Description
Rabbit polyclonal to BMX
Specificity
ab59360 detects endogenous levels of total BMX protein.
Tested applications
ELISA, ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized non phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).
Positive control
A549 cytoplasmic lysate (WB) Human thyroid gland tissue (IHC)
General notes
Store at -20°C for 1 year
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
ICC/IF: Use at an assay dependent dilution.
WB: 1/500 - 1/1000.Detects a band of approximately 78 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.
Tissue specificity
Preferentially expressed in epithelial and endothelial cells.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Domain
SH2 domain mediates interaction with RUFY1.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP51813
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bone marrow tyrosine kinase gene in chromosome X protein antibody
- Bmx antibody
- BMX non receptor tyrosine kinase antibody
see all
Database links
- Entrez Gene: 660 Human
- Entrez Gene: 12169 Mouse
- Omim: 300101 Human
- SwissProt: P51813 Human
- SwissProt: P97504 Mouse
- Unigene: 495731 Human
- Unigene: 504 Mouse
Anti-BMX antibody images:
Western blot - BMX antibody (ab59360)
All lanes : Anti-BMX antibody (ab59360) at 1/500 dilution
Lane 1 : Extracts from A549 cells with no immunizing peptide
Lane 2 : Extracts from A549 cells with immunizing peptide
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - BMX antibody (ab59360)
ab59360, at a 1/50 dilution, staining human BMX in thyroid gland, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
Immunocytochemistry/ Immunofluorescence-BMX antibody(ab59360)
ICC/IF image of ab59360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-BMX antibody (ab59360)
This product has been referenced in:
- Guryanova OAet al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 21481791) »
See 1 publication for this product
Publishing research using ab59360? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"