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Western blot - BMX antibody (ab59360)
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Immunohistochemistry (Paraffin-embedded sections) - BMX antibody (ab59360)
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Immunocytochemistry/ Immunofluorescence-BMX antibody(ab59360)
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Product Name
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BMX antibody
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See all BMX antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BMX
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Immunogen
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Synthesized non phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab59360 detects endogenous levels of total BMX protein.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A549 cytoplasmic lysate (WB)
Human thyroid gland tissue (IHC)
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Tyrosine kinases are either receptor molecules, which contain transmembrane and extracellular domains, or nonreceptor proteins, which are located intracellularly. BMX[ETK] is a member of Bruton's tyrosine kinase family. It is expressed in haematopoetic, epithelial and endothelial cells and participates in multiple signal transduction pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Purified from rabbit antiserum by affinity chromatography, using
epitope specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Store at -20°C for 1 year
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMX antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMX antibody images, references, products related to ab59360 and other tools.
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BMX antibody images:
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 Western blot - BMX antibody (ab59360)
All lanes : BMX antibody (ab59360) at 1/500 dilution
Lane 1 : Extracts from A549 cells with no immunizing peptide
Lane 2 : Extracts from A549 cells with immunizing peptide
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Paraffin-embedded sections) - BMX antibody (ab59360)
ab59360, at a 1/50 dilution, staining human BMX in thyroid gland, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).
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Immunocytochemistry/ Immunofluorescence-BMX antibody(ab59360)
ICC/IF image of ab59360 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to BMX
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BMX antibody - more information
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Customer reviews (feedback) regarding BMX antibody |
Customer FAQs regarding BMX antibody |
Protocols for BMX antibody |
Price and availability of products related to BMX antibody |
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BMX antibody - related products:
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BMX antibody - other tools:
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Contact Abcam with a Technical Enquiry about BMX antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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