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Anti-BMX antibody (ab73887)

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Overview

Product name

Anti-BMX antibody
See all BMX products (8) ...

Description

Rabbit polyclonal to BMX

Tested applications

ELISA, IP, WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide derived from the N-terminal region of human BMX

Positive control

WB: extracts from COS-7 cells; IHC-P: human thyroid gland tissue; ICC/IF: A549 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other

  • Western blot - BMX antibody (ab73887)Western blot - BMX antibody (ab73887) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887)Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab73887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent concentration. (PubMed: 21788516)

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 78 kDa.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/1000.

    IHC-P: 1/50 - 1/1000.

  • 1 Image

    ICC/IF

     ICC/IF: 1/500 - 1/1000.

    ICC/IF: 1/500 - 1/1000.

Target

Function

Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Tissue specificity

Preferentially expressed in epithelial and endothelial cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.

Domain

SH2 domain mediates interaction with RUFY1.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP51813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bone marrow tyrosine kinase gene in chromosome X protein antibody
  • Bmx antibody
  • BMX non receptor tyrosine kinase antibody
  • BMX_HUMAN antibody
  • Cytoplasmic tyrosine protein kinase BMX antibody
  • Cytoplasmic tyrosine-protein kinase BMX antibody
  • Epithelial and endothelial tyrosine kinase antibody
  • ETK antibody
  • NKT38 antibody
  • NTK 38 antibody
  • NTK38 antibody
  • Protein tyrosine kinase BMX antibody
  • PSCTK 2 antibody
  • PSCTK 3 antibody
  • PSCTK2 antibody
  • PSCTK3 antibody
see all

Database links

Anti-BMX antibody images:

  Western blot - BMX antibody (ab73887)

Western blot - BMX antibody (ab73887)

All lanes : Anti-BMX antibody (ab73887) at 1/500 dilution

Lane 1 : extracts from COS7 cells
Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 78 kDa
Observed band size : 78 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

  Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887)

Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887)

Left panel: immunofluorescence analysis of A549 cells, using 1/550-1/1000 ab73887. Right panel: same, after pre-incubation with the immunizing peptide.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887)

Left panel: immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using 1/50-1/100 ab73887; Right panel: same, after pre-incubation with the immunizing peptide.

References for Anti-BMX antibody (ab73887)

This product has been referenced in:

  • Hart JRet al. Essential role of Stat3 in PI3K-induced oncogenic transformation. Proc Natl Acad Sci U S A 108:13247-52 (2011). WB, IP; Mouse.Read more (PubMed: 21788516) »
  • Lage Ket al. Dissecting spatio-temporal protein networks driving human heart development and related disorders. Mol Syst Biol 6:381 (2010). IHC-P; Human.Read more (PubMed: 20571530) »

See all 2 publications for this product

Publishing research using ab73887? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"