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Western blot - BMX antibody (ab73887)
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Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887)
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Product Name
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BMX antibody
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See all BMX antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BMX
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Immunogen
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Synthetic peptide derived from the N-terminal region of human BMX
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/1000.
WB: 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: extracts from COS-7 cells;
IHC-P: human thyroid gland tissue;
ICC/IF: A549 cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Tyrosine kinases are either receptor molecules, which contain transmembrane and extracellular domains, or nonreceptor proteins, which are located intracellularly. BMX[ETK] is a member of Bruton's tyrosine kinase family. It is expressed in haematopoetic, epithelial and endothelial cells and participates in multiple signal transduction pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMX antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMX antibody images, references, products related to ab73887 and other tools.
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BMX antibody images:
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 Western blot - BMX antibody (ab73887)
All lanes : BMX antibody (ab73887) at 1/500 dilution
Lane 1 : extracts from COS7 cells
Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 78 kDa
Observed band size : 78 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - BMX antibody (ab73887)
Left panel: immunofluorescence analysis of A549 cells, using 1/550-1/1000 ab73887. Right panel: same, after pre-incubation with the immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BMX antibody (ab73887)
Left panel: immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using 1/50-1/100 ab73887; Right panel: same, after pre-incubation with the immunizing peptide.
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Search PubMed (MEDLINE) for references to BMX
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BMX antibody - more information
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Customer reviews (feedback) regarding BMX antibody |
Customer FAQs regarding BMX antibody |
Protocols for BMX antibody |
Price and availability of products related to BMX antibody |
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BMX antibody - related products:
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BMX antibody - other tools:
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Contact Abcam with a Technical Enquiry about BMX antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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