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Anti-BMX (phospho Y566) antibody (ab59409)

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Overview

Product name

Anti-BMX (phospho Y566) antibody
See all BMX products (8) ...

Description

Rabbit polyclonal to BMX (phospho Y566)

Specificity

This antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566.

Tested applications

IHC-P, ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).

Epitope

Human: Tyr566; Mouse: Tyr542

Positive control

Human thyroid gland tissue

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other

  • Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409)Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409) image (enlarge)

  • Western blot - BMX (phospho Y566) antibody (ab59409)Western blot - BMX (phospho Y566) antibody (ab59409) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab59409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

  • ShowHide1 Image

    WB

     WB: 1/3000Detects a band...Read more →

    WB: 1/3000Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).

Target

Function

Activity is required for interleukin 6 (IL-6) induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Tissue specificity

Preferentially expressed in epithelial and endothelial cells.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.

Domain

SH2 domain mediates interaction with RUFY1.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP51813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bone marrow tyrosine kinase gene in chromosome X protein antibody
  • Bmx antibody
  • BMX non receptor tyrosine kinase antibody
  • BMX_HUMAN antibody
  • Cytoplasmic tyrosine protein kinase BMX antibody
  • Cytoplasmic tyrosine-protein kinase BMX antibody
  • Epithelial and endothelial tyrosine kinase antibody
  • ETK antibody
  • NKT38 antibody
  • NTK 38 antibody
  • NTK38 antibody
  • Protein tyrosine kinase BMX antibody
  • PSCTK 2 antibody
  • PSCTK 3 antibody
  • PSCTK2 antibody
  • PSCTK3 antibody
see all

Database links

Anti-BMX (phospho Y566) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409)

Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409)

Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.

  Western blot - BMX (phospho Y566) antibody (ab59409)

Western blot - BMX (phospho Y566) antibody (ab59409)

Anti-BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 78 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)

References for Anti-BMX (phospho Y566) antibody (ab59409)

This product has been referenced in:

  • Guryanova OAet al. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3. Cancer Cell 19:498-511 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 21481791) »

See 1 publication for this product

Publishing research using ab59409? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"