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Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409)
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Western blot - BMX (phospho Y566) antibody (ab59409)
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Product Name
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BMX (phospho Y566) antibody
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See all BMX antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BMX (phospho Y566)
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Immunogen
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Synthetic phosphopeptide derived from human BMX around the phosphorylation site of tyrosine 566 (DQYPVS).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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This antibody detects endogenous levels of ETK only when phosphorylated at tyrosine 566.
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Epitope
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Human: Tyr566; Mouse: Tyr542
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human thyroid gland tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Tyrosine kinases are either receptor molecules, which contain transmembrane and extracellular domains, or nonreceptor proteins, which are located intracellularly. BMX[ETK] is a member of Bruton's tyrosine kinase family. It is expressed in haematopoetic, epithelial and endothelial cells and participates in multiple signal transduction pathways.
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BMX (phospho Y566) antibody (ab59409) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BMX (phospho Y566) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BMX (phospho Y566) antibody images, references, products related to ab59409 and other tools.
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BMX (phospho Y566) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - BMX (phospho Y566) antibody (ab59409)
Immunohistochemical analysis of paraffin embedded human thyroid gland tissue using ab59409 at a 1/50 dilution, in the presence (right) and absence (left) of immunizing phosphopeptide.
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Western blot - BMX (phospho Y566) antibody (ab59409)
BMX (phospho Y566) antibody (ab59409) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 78 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to BMX
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BMX (phospho Y566) antibody - more information
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Customer reviews (feedback) regarding BMX (phospho Y566) antibody |
Customer FAQs regarding BMX (phospho Y566) antibody |
Protocols for BMX (phospho Y566) antibody |
Price and availability of products related to BMX (phospho Y566) antibody |
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BMX (phospho Y566) antibody - related products:
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BMX (phospho Y566) antibody - other tools:
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Contact Abcam with a Technical Enquiry about BMX (phospho Y566) antibody |
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