Recombinant Anti-BNIP1 antibody [EPR10024] (ab151551)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10024] to BNIP1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BNIP1 antibody [EPR10024]
See all BNIP1 primary antibodies -
Description
Rabbit monoclonal [EPR10024] to BNIP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- C6, L6, PC-12, A431, Ramos, HepG2 and Jurkat cell lysates; Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10024 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
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Function
SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. -
Tissue specificity
Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine. -
Sequence similarities
Belongs to the SEC20 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 662 Human
- Entrez Gene: 224630 Mouse
- Entrez Gene: 140932 Rat
- Omim: 603291 Human
- SwissProt: Q12981 Human
- SwissProt: Q6QD59 Mouse
- SwissProt: Q8VHI8 Rat
- Unigene: 145726 Human
see all -
Alternative names
- BCL2 adenovirus E1B 19kD interacting protein 1 antibody
- BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 antibody
- BNIP1 antibody
see all
Images
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All lanes : Anti-BNIP1 antibody [EPR10024] (ab151551) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : L6 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling BNIP1 with ab151551 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151551 has been referenced in 1 publication.
- Sun D et al. Fluoxetine induces autophagic cell death via eEF2K-AMPK-mTOR-ULK complex axis in triple negative breast cancer. Cell Prolif 51:e12402 (2018). PubMed: 29094413