Anti-BNIP3 antibody [ANa40] (HRP) (ab196707)

Overview

  • Product name
    Anti-BNIP3 antibody [ANa40] (HRP)
    See all BNIP3 primary antibodies
  • Description
    Mouse monoclonal [ANa40] to BNIP3 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human BNIP3 aa 1 to the C-terminus.

  • Epitope
    The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.
  • Positive control
    • WB: Human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab196707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.5 kDa).

Target

  • Function
    Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
  • Sequence similarities
    Belongs to the NIP3 family.
  • Cellular localization
    Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
    • BNIP 3 antibody
    • BNIP3 antibody
    • BNIP3_HUMAN antibody
    • NIP 3 antibody
    • NIP3 antibody
    see all

Images

  • Anti-BNIP3 antibody [ANa40] (HRP) (ab196707) at 1/5000 dilution + Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21.5 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196707 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab196707 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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