Anti-BNIP3 antibody [ANa40] (ab10433)

Product name

Anti-BNIP3 antibody [ANa40]
See all BNIP3 products (7) ...

Description

Mouse monoclonal [ANa40] to BNIP3

Specificity

This antibody was originally tested on whole cell lysate from 293T cells transfected with BNIP3. Levels of constitutively expressed BNIP3 are very variable and a good positive control is strongly recommended. Zhang et aln (2008) comment "Very low levels of BNIP3 protein were detected by immunoblot assay of lysates prepared from WT MEFs that were cultured at 20% O2, whereas hypoxia strongly induced expression of BNIP3 protein, which migrated as a 30-kDa monomer and 60-kDa dimer, as previously described. ... The expression of BNIP3 mRNA and protein was significantly reduced in the lungs of HET (as compared with WT) and Cre+ (as compared with Cre-) mice, demonstrating that BNIP3 expression is regulated by HIF-1 under physiological conditions in vivo."

Tested applications

ICC, ELISA, IP, WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Epitope

The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Positive control

Whole cell lysate from 293T cells transfected with BNIP3.

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Primary antibody notes

BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein. Homologues of BNIP3 sharing both structural and functional similarity have been identified in mammals: Nix (also called BNIP3L/BNIP3alpha\/B5) and, in C. elegans, ceBNIP3. The TM domain of BNIP3 and Nix share 80% identity. Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction characterized by opening of the mitochondrial permeability transition (PT) pore, proton electrochemical gradient suppression, and increased reactive oxygen species production. BNIP3 has been reported to localize to the nuclear envelope when co-expressed with E1B 19K. Endogenous BNIP3 protein is abundant in murine and human skeletal muscle and is not detectable in lysates of all other nonskeletal muscle-bearing tissues and many cell lines, including myoblasts and differentiated myocytes.

Clonality

Monoclonal

Clone number

ANa40

Myeloma

unknown

Isotype

IgG2b

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Heart >> Apoptosis
Cell Biology >> Apoptosis >> Mitochondrial
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family