Anti-BNIP3 antibody - Carboxyterminal end (ab65874)
Key features and details
- Rabbit polyclonal to BNIP3 - Carboxyterminal end
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BNIP3 antibody - Carboxyterminal end
See all BNIP3 primary antibodies -
Description
Rabbit polyclonal to BNIP3 - Carboxyterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide:
AIGLGIYIGRRLTTSTST
corresponding to a sequence near the C-terminal of human BNIP3, identical to the related mouse and rat sequence. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa).
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30, 60 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. -
Sequence similarities
Belongs to the NIP3 family. -
Cellular localization
Mitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. - Information by UniProt
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Database links
- Entrez Gene: 664 Human
- Entrez Gene: 84480 Rat
- Omim: 603293 Human
- SwissProt: Q12983 Human
- Unigene: 144873 Human
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Alternative names
- BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
- BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
- BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
see all
Images
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Anti-BNIP3 antibody - Carboxyterminal end (ab65874) at 2 µg/ml + rat thymus tissue lysate at 30 µg
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible dimer) -
ICC/IF image of ab65874 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65874, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab65874 has been referenced in 5 publications.
- Zhu L et al. BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice. Int Immunopharmacol 87:106799 (2020). PubMed: 32717566
- Salem AF et al. Cigarette smoke metabolically promotes cancer, via autophagy and premature aging in the host stromal microenvironment. Cell Cycle 12:818-25 (2013). PubMed: 23388463
- Fedorova LV et al. Peroxisome proliferator-activated receptor d agonist, HPP593, prevents renal necrosis under chronic ischemia. PLoS One 8:e64436 (2013). IHC-P ; Rat . PubMed: 23691217
- Fedorova LV et al. Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach. BMC Nephrol 14:209 (2013). WB ; Rat . PubMed: 24090408
- Kalas W et al. H-ras Up-regulates Expression of BNIP3. Anticancer Res 31:2869-75 (2011). WB ; Human . PubMed: 21868531