Anti-BNIP3 antibody (ab38621)

Overview

• Product nameAnti-BNIP3 antibodySee all BNIP3 primary antibodies ...
• Description
  Rabbit polyclonal to BNIP3
• Tested applicationsWB, ELISA, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  KLH conjugated synthetic peptide, selected from within aa 1-100 (BH3 domain) of human NIP3.

• Positive controlMouse brain tissue lysate and Ramos cell lysate, breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cardiovascular
  • Heart
  • Apoptosis

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
• Sequence similaritiesBelongs to the NIP3 family.
• Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.

Target information above from: UniProt accession Q12983 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 664 Human
  • Entrez Gene: 12176 Mouse
  • Omim: 603293 Human
  • SwissProt: Q12983 Human
  • SwissProt: O55003 Mouse
  • Unigene: 144873 Human
  • Unigene: 378890 Mouse

• Alternative names
  BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibodyBCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibodyBCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody

  BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibodyBNIP 3 antibodyBNIP3 antibodyBNIP3_HUMAN antibodyNIP 3 antibodyNIP3 antibodyNIP3 antibody

  see all

Anti-BNIP3 antibody images

• 

  Western blot - BNIP3 antibody (ab38621)
  All lanes : Anti-BNIP3 antibody (ab38621) at 1/100 dilution
  
  Lane 1 : Ramos cell lysate
  Lane 2 : Mouse brain tissue lysate
  
  
  Predicted band size : 22 kDa
  Observed band size : 22 kDa
  Additional bands at : 15 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BNIP3 antibody (ab38621)
  This image shows human breast carcinoma stained with ab38621 at 1/50 dilution.
• 

  Immunohistochemistry (Paraffin-embedded sections) - BNIP3 antibody (ab38621)
  This image shows human colon carcinoma stained with ab38621 at 5ug/200ul dilution.
• 

  Immunocytochemistry/ Immunofluorescence-BNIP3 antibody(ab38621)
  ICC/IF image of ab38621 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38621, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

Protocols

• Recommended IHC-P protocol with ab38621