Overview

  • Product nameAnti-BNIP3 antibodySee all BNIP3 primary antibodies ...
  • Description
    Rabbit polyclonal to BNIP3
  • Tested applicationsWB, ELISA, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide, selected from within aa 1-100 (BH3 domain) of human NIP3.

  • Positive control
    • Mouse brain tissue lysate and Ramos cell lysate, breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab38621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/100 - 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionApoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
  • Sequence similaritiesBelongs to the NIP3 family.
  • Cellular localizationMitochondrion. Mitochondrion membrane. Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria.
  • Target information above from: UniProt accession Q12983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody
    • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody
    • BNIP 3 antibody
    • BNIP3 antibody
    • BNIP3_HUMAN antibody
    • NIP 3 antibody
    • NIP3 antibody
    • NIP3 antibody
    see all

Anti-BNIP3 antibody images

  • All lanes : Anti-BNIP3 antibody (ab38621) at 1/100 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Mouse brain tissue lysate


    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 15 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • This image shows human breast carcinoma stained with ab38621 at 1/50 dilution.
  • This image shows human colon carcinoma stained with ab38621 at 5ug/200ul dilution.
  • ICC/IF image of ab38621 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38621, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BNIP3 antibody (ab38621)

This product has been referenced in:
  • Millar NL  et al. Hypoxia: a critical regulator of early human tendinopathy. Ann Rheum Dis 71:302-10 (2012). IHC-P ; Human . Read more (PubMed: 21972243) »
  • Wu SY  et al. Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation. Neoplasia 13:1171-82 (2011). WB ; Human . Read more (PubMed: 22241963) »

See all 6 Publications for this product

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