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Product name
Anti-BNIP3L antibody
See all BNIP3L products (8) ...
Description
Rabbit polyclonal to BNIP3L
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
peptide (DAQHESGQSSSRGSSH) corresponding to amino acids 77 to 92 of human origin,which are identical to those of mouse Bnip3L (3).
DAQHESGQSSSRGSSH
Positive control
K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L (4).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitophagy fission and fusion
Cardiovascular >> Heart >> Apoptosis
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> p53 Pathway
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa.To reduce possible background load less sample and or use more diluted antibody.
ICC/IF: Use at an assay dependent dilution. See Abreview.
IHC-P: Use a concentration of 2 µg/ml.
Target
Function
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3.
Sequence similarities
Belongs to the NIP3 family.
Post-translational
modifications
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.
Cellular localization
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane.
Target information above from: UniProt accessionO60238
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
Database links
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Omim: 605368 Human
- SwissProt: O60238 Human
- SwissProt: Q9Z2F7 Mouse
- Unigene: 131226 Human
- Unigene: 29820 Mouse
- Unigene: 347081 Mouse
Anti-BNIP3L antibody images:
Western blot - BNIP3L antibody (ab8399)
All lanes : Anti-BNIP3L antibody (ab8399) at 1 µg/ml
Lane 1 : K562 whole cell lysate with absence of immunogenic peptide
Lane 2 : K562 whole cell lysate with presence of immunogenic peptide
The approximate 40 kDa top band represents monomer of Bnip3L.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BNIP3L antibody (ab8399)
ab8399 at 2µg/ml staining BNIP3L in human kidney tissue by IHC
Immunocytochemistry/ Immunofluorescence - BNIP3L antibody (ab8399)
ab8399 staining BNIP3L (NIX) in Mouse MEF cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X100 and blocked with 10% Horse serum in 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500: in 10% Horse serum in 5% BSA ) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-BNIP3L antibody (ab8399)
This product has been referenced in:
- Li D et al. Restrictive loss of plakoglobin in cardiomyocytes leads to arrhythmogenic cardiomyopathy. Hum Mol Genet 20:4582-96 (2011).Read more (PubMed: 21880664) »
- Pineau P et al. miR-221 overexpression contributes to liver tumorigenesis. Proc Natl Acad Sci U S A 107:264-9 (2010). WB; Human.Read more (PubMed: 20018759) »
See all 6 publications for this product
Publishing research using ab8399? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"