Overview

  • Product nameBNP 32 Rat ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.06 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s BNP 32 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 32 in plasma, serum, tissue extract, and cell culture supernatants.

    A BNP 32 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 32 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 32 captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificityBrain and also in atria, but at much lower levels than ANP.
  • Sequence similaritiesBelongs to the natriuretic peptide family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Gamma-brain natriuretic peptide
    • nppb
    see all

Applications

Our Abpromise guarantee covers the use of ab108815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

BNP 32 Rat ELISA Kit images

  • Representative Standard Curve using ab108815.

Protocols

References for BNP 32 Rat ELISA Kit (ab108815)

ab108815 has not yet been referenced specifically in any publications.

Product Wall



Le laboratoire m’a confirmé que vous pouvez utiliser un tampon EDTA plutôt qu’un tampon de citrate pour vos échantillons de plasma.

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet êtr...

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Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Cou...

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Thank you for your enquiry.

I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

According to a litera...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"