BNP 32 Rat ELISA Kit (ab108815)
- Product nameBNP 32 Rat ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Tissue
- Assay typeSandwich
- Sensitivity= 0.03 ng/ml
- Range0.031 ng/ml - 2 ng/ml
- Assay time5h 00m
- Species reactivityReacts with: Rat
- Product overview
Natriuretic peptides (ANP, BNP and CNP) comprise a family of structurally related peptides, which are derived from three different genes and share a 17-amino acid internal ring. A high level of plasma BNP may have a strong, independent association with increased mortality rates in patients with primary pulmonary hypertension (PPH), congestive heart failure and/or after acute myocardial infarction.
ab108815 BNP 32 Rat ELISA kit is designed for detection of rat BNP 32 (rBNP 32) in plasma, serum, tissue extract and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rBNP 32 in less than 5 hours. A polyclonal antibody specific for rBNP 32 has been pre-coated onto a microplate. The rBNP 32 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rBNP 32, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated rBNP-32 Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml rBNP-32 Microplate 1 unit rBNP-32 Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
Our Abpromise guarantee covers the use of ab108815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for BNP 32 Rat ELISA Kit (ab108815)
ab108815 has not yet been referenced specifically in any publications.