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Overview

  • Product nameBNP 32 Rat ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich
  • Sensitivity
    = 0.03 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Natriuretic peptides (ANP, BNP and CNP) comprise a family of structurally related peptides, which are derived from three different genes and share a 17-amino acid internal ring. A high level of plasma BNP may have a strong, independent association with increased mortality rates in patients with primary pulmonary hypertension (PPH), congestive heart failure and/or after acute myocardial infarction.

    ab108815 BNP 32 Rat ELISA kit is designed for detection of rat BNP 32 (rBNP 32) in plasma, serum, tissue extract and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rBNP 32 in less than 5 hours. A polyclonal antibody specific for rBNP 32 has been pre-coated onto a microplate. The rBNP 32 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rBNP 32, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

Applications

Our Abpromise guarantee covers the use of ab108815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA

BNP 32 Rat ELISA Kit images

  • ELISA - BNP 32 Rat ELISA Kit (ab108815)
    ELISA - BNP 32 Rat ELISA Kit (ab108815)

Protocols

References for BNP 32 Rat ELISA Kit (ab108815)

ab108815 has not yet been referenced specifically in any publications.

Publishing research using ab108815 ? Please let us know so that we can cite the reference in this datasheet

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Votes: Highest First

Young rat blood samples.
Is the kit ab108815 and/or ab108816 suitable for plasma obtained from blood collected using heparin tubes, centrifuged and stored at -80ºC?
Also, what is the difference between the BNP-32 and BNP-45 ?

Merci de nous avoir contactés.

Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

1) Les deux kits peuvent en effet être u...

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Inquiry: Have you got data concerning the accuracy of the kit below : Ab108815 BNP 32 Rat ELISA KIT Ab108797 ANP Rat ELISA KIT In house we have performed a validation but the % of accuracies are between 11.6 % and 80.6% for ANP Rat ELISA KIT and 63.2 % an...

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Thank you for your enquiry regarding ab108797 and ab108815. I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

It is hard to answer the question without any detail information. Could ...

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Dear Technical Service of Abcam,

We are very interested in your BNP ELSA for rats (cat# ab108815 or ab108816).

Can you please explain me the difference between the BNP 45 Rat ELISA kit (ab108816) and the BNP 32 Rat ELISA kit (ab10881...

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Thank you for your enquiry.

I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

According to a literatur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"