You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameBNP 45 Rat ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell culture extracts
  • Assay typeSandwich
  • Sensitivity
    = 0.03 ng/ml
  • Range
    0.031 ng/ml - 1 ng/ml
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Natriuretic peptides (ANP, BNP and CNP) comprise a family of structurally related peptides, which are derived from three different genes and share a 17-amino acid internal ring. A high level of plasma BNP may have a strong, independent association with increased mortality rates in patients with primary pulmonary hypertension (PPH), congestive heart failure and/or after acute myocardial infarction

    ab108816 is designed for detection of rat BNP 45 in plasma, serum, tissue extract, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures rat BNP 45 in less than 5 hours. A polyclonal antibody specific for rat BNP 45 has been pre-coated onto a microplate. The rat BNP 45 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for rat BNP 45, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108816 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      BNP 45 Rat ELISA Kit images

      • ELISA - BNP 45 Rat ELISA Kit (ab108816)
        ELISA - BNP 45 Rat ELISA Kit (ab108816)

      Protocols

      References for BNP 45 Rat ELISA Kit (ab108816)

      This product has been referenced in:
      • Wang YB  et al. Rehmannia glutinosa extract activates endothelial progenitor cells in a rat model of myocardial infarction through a SDF-1 a/CXCR4 cascade. PLoS One 8:e54303 (2013). ELISA ; Rat . Read more (PubMed: 23349848) »

      See 1 Publication for this product

      Publishing research using ab108816 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108816 Submit a Question for ab108816

      Displaying 1 - 10 of 10 results for Abreviews and Q&A

      10
      Votes: Highest First

      Young rat blood samples.
      Is the kit ab108815 and/or ab108816 suitable for plasma obtained from blood collected using heparin tubes, centrifuged and stored at -80ºC?
      Also, what is the difference between the BNP-32 and BNP-45 ?

      Merci de nous avoir contactés.

      Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

      1) Les deux kits peuvent en effet être u...

      Read More

      sorry I made a typo. My question refers to kit no. ab 108816 (BNB-45 detection in rats).

      Could you give me information asap about the need of fasted/non-fasted serum samples?

      Many thanks in advance,

      My colleague Tanya is out of office today so I am dealing with her inquires.

      I can confirm that the sample needs not to be from fasted animals. Regular samples will be fine.

      Thanks!

      Is it ok to keep on feeding the rats prior to the experiments?

      Vielen Dank für Ihren Anruf.

      Wenn das Futter keinen Einfluss auf die Produktion dieser Peptide hat (wie durch zB salzreiche Nahrung), sehe ich keinen Grund, warum die Ratten fasten sollten.

      Ich hoffe, dies hilft Ihnen weiter. Bi...

      Read More

      I received the kit ab108816 (BNP 45 Rat ELISA kit) and it was improperly stored in the freezer for two days, it can change its performance? I will use it on Tuesday.

      Thank you

      Thank you for contacting us.

      The best way to test the viability is using the kit. The kits should be fine at -20C so please use the kit without any hesitation.

      I hope this information is helpful to you. Please do not hesitate to contact ...

      Read More


      The answers followed below:
      1) The sample collected to quantify the concentration of BNP was the
      blood plasma of Wistar rats.
      After the samples
      had been collected it was transferred to a test tube suitable for the
      volume coll...

      Read More

      Thank you for your reply and the additional information.

      We have the following PO on file: xxx.The order was from xxx and the lot number matches what you had sent, thus I think this is the correct order.


      I have now arranged the ...

      Read More

      Our customer complained about a Abcam kit that she bought.
      She bought two BNP 45 kit for rat (ab108816). The first one, lote
      GR53511-2 , worked well. The second kit was the problem, lote
      GR53511-4. First of all, there was missing the standa...

      Read More

      Thank you for contacting us.

      I am very sorry to hear about the problems your customer had to face because of the second kit. I sincerely apologize about that! Yes, I'd be happy to arrange for a free of charge replacement.


      Could ...

      Read More

      Buenos dias,
      Adjunto una foto de dos viales de estandar del kit recibidos en dos pedidos diferentes.
      En el segundo pedido no se especificaba la cantidad de estandar contenida.
      Podrian por favor indicarnos de que cantidad se trata?
      Much...

      Read More

      Gracias por contactarnos y alertarnos de este fallo.



      Lamento mucho que el vial de estándar del nuevo lote enviado no indicara ningún dato acerca de la cantidad contenida. Sin duda se trata de un error que estamos tratando...

      Read More

      Hello tech support team! How are you?



      I have a customer enquiring about finding a similar antibody to AB108816 that would react with Human instead of Rat.



      Could you please advise us whether there’s such ...

      Read More

      Thank you for your enquiry.

      Currently, this is the only BNP 45 ELISA kit (ab108816) which recognizes rat BNP 45. We sell primary antibodies which detect human BNP:

      ab20984,

      ab19645,

      ab19646,

      ab47699,
      ...

      Read More

      Could you please clarify me for how long should I incubate the chromogen substrate? 10 min or 15 min? -> protocol, page 4: Add 50 μl of Chromogen Substrate to each well. Incubate 10 minutes or till the optimal blue colour density develops. -> pro...

      Read More

      Thank you for your enquiry and your interest.

      As the Protocol booklet indicates we would suggest adding 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue colour density develops. The optimal time...

      Read More

      Dear Technical Service of Abcam,

      We are very interested in your BNP ELSA for rats (cat# ab108815 or ab108816).

      Can you please explain me the difference between the BNP 45 Rat ELISA kit (ab108816) and the BNP 32 Rat ELISA kit (ab10881...

      Read More

      Thank you for your enquiry.

      I can confirm that the first difference between these kits is that ab108815 detects the 32 amino acid form of BNP, andab108816 detects the larger 45 amino acid form of the protein.

      According to a literatur...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"