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Anti-BOB1 antibody (ab3426)

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Overview

Product name

Anti-BOB1 antibody
See all BOB1 products (9) ...

Description

Goat polyclonal to BOB1

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse

Predicted to work with

Human

Immunogen

Synthetic peptide: DAYALNHTLSVEGF, corresponding to C terminal amino acids 243-256 of Human BOB1.

C-DAYALNHTLSVEGF

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Mouse spleen lysate.

General notes

Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3

Concentration

Concentration information loading...

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> POU

Immunology >> Adaptive Immunity >> B Cells >> Non-CD

  • Western blot - BOB1 antibody (ab3426)Western blot - BOB1 antibody (ab3426) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa in Mouse Spleen lysate.
(predicted molecular weight: 28 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Tissue specificity

B-cell specific.

Involvement in disease

Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

Sequence similarities

Belongs to the POU2AF1 family.

Post-translational
modifications

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ16633 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B cell Oct binding protein 1 antibody
  • B cell specific coactivator OBF 1 antibody
  • B cell specific coactivator OBF1 antibody
  • B-cell-specific coactivator OBF-1 antibody
  • BOB 1 antibody
  • BOB-1 antibody
  • OBF 1 antibody
  • OBF1 antibody
  • OBF1_HUMAN antibody
  • OCA B antibody
  • OCA-B antibody
  • OCAB antibody
  • OCT binding factor 1 antibody
  • OCT-binding factor 1 antibody
  • POU domain class 2 associating factor 1 antibody
  • POU domain class 2-associating factor 1 antibody
  • POU2AF1 antibody
see all

Database links

Anti-BOB1 antibody images:

  Western blot - BOB1 antibody (ab3426)

Western blot - BOB1 antibody (ab3426)



Predicted band size : 28 kDa


ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).

References for Anti-BOB1 antibody (ab3426)

ab3426 has not yet been referenced specifically in any publications.

Publishing research using ab3426? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"