Recombinant Anti-BORIS antibody [EP12204] (ab187163)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP12204] to BORIS
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BORIS antibody [EP12204]
See all BORIS primary antibodies -
Description
Rabbit monoclonal [EP12204] to BORIS -
Host species
Rabbit -
Specificity
We don't recommend WB application for this antibody as it detects extra bands.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, mouse and rat testis tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP12204 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. -
Tissue specificity
Testis specific. Specifically expressed in primary spermatocytes. -
Sequence similarities
Belongs to the CTCF zinc-finger protein family.
Contains 11 C2H2-type zinc fingers. -
Developmental stage
Is up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation). -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 140690 Human
- Entrez Gene: 664799 Mouse
- Entrez Gene: 100360757 Rat
- Omim: 607022 Human
- SwissProt: Q8NI51 Human
- SwissProt: A2APF3 Mouse
- Unigene: 131543 Human
- Unigene: 389396 Mouse
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Alternative names
- BORIS like protein antibody
- Brother of the regulator of imprinted sites antibody
- Cancer/testis antigen 27 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab187163 has been referenced in 3 publications.
- Gong M et al. Genome-wide bioinformatics analysis reveals CTCFL is upregulated in high-grade epithelial ovarian cancer. Oncol Lett 18:4030-4039 (2019). PubMed: 31516605
- Debruyne DN et al. BORIS promotes chromatin regulatory interactions in treatment-resistant cancer cells. Nature 572:676-680 (2019). PubMed: 31391581
- López-Romero R et al. The expression of transcription factor BORIS and its association with the estrogen receptor beta (ER-ß) in cervical carcinogenesis. Int J Clin Exp Pathol 12:3208-3221 (2019). PubMed: 31934165