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Overview

  • Product nameBORIS peptideSee all BORIS proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 1 - 100 of Human BORIS. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab18337 - BORIS antibody - ChIP Grade.
  • Protein length663 amino acids

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab22203 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml.
    This peptide can be used with studies using ab18337.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        BORIS like proteinBrother of the regulator of imprinted sitesCancer/testis antigen 27
        CCCTC binding factor (zinc finger protein) likeCCCTC-binding factorCT27CTCF paralogCTCF TCTCF-like proteinCTCFLCTCFL_HUMANdJ579F20.2HMG 1L1HMGB1L1MGC163358MGC169105MGC169106Putative high mobility group protein 1 like 1Putative high mobility group protein B1 like 1Transcriptional repressor CTCFLZinc finger protein CTCF-T
      see all
  • FunctionTestis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
  • Tissue specificityTestis specific. Specifically expressed in primary spermatocytes.
  • Sequence similaritiesBelongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Developmental stageIs up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation).
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q8NI51 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for BORIS peptide (ab22203)

    This product has been referenced in:
    • Woloszynska-Read A  et al. Coordinated cancer germline antigen promoter and global DNA hypomethylation in ovarian cancer: association with the BORIS/CTCF expression ratio and advanced stage. Clin Cancer Res 17:2170-80 (2011). Blocking . Read more (PubMed: 21296871) »
    • Jones TA  et al. Widespread Expression of BORIS/CTCFL in Normal and Cancer Cells. PLoS One 6:e22399 (2011). Blocking . Read more (PubMed: 21811597) »

    See all 2 Publications for this product

    Publishing research using ab22203 ? Please let us know so that we can cite the reference in this datasheet

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    Two months ago we order antibodies against CTCF and BORIS. We tested them and we were disappointed by the data we obtained. We performed IHC on fixed testicular tissues and we compared the results we obtained with those obtained with homemade antibodies d...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with our CTCF antibody (ab10571). Please can you tell me whether you have been applying this antibody using formalin fixed tissue (IHC-P) or using perfusion fixed fr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"