Overview
- Product nameAnti-BPGM antibodySee all BPGM primary antibodies ...
- DescriptionRabbit polyclonal to BPGM
- Tested applicationsWB more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 229-259 of Human BPGM (NP_001715.1 , NP_954655.1).
- Positive controlMDA-MB-435 cell lysate; 293 cell lysate transiently transfected with BPGM
Properties
- FormLiquid
- Storage instructionsStore at 4°C (up to 6 months). For long term storage store at -20°C
- Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab112957 was purified through a protein A column, followed by peptide affinity purification.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab112957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/100 - 1/500. Predicted molecular weight: 30 kDa. |
Target
- FunctionPlays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities.
- Involvement in diseaseDefects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis.
- Sequence similaritiesBelongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
- Post-translational
modificationsGlycation of Lys-159 in diabetic patients inactivates the enzyme. -
Database links
- Entrez Gene: 669 Human
- Omim: 222800 Human
- SwissProt: P07738 Human
- Unigene: 198365 Human
Target information above from: UniProt accession
P07738
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- 2 antibody2,3-bisphosphoglycerate mutase antibody2,3-bisphosphoglycerate mutase, erythrocyte antibody
- 2,3-bisphosphoglycerate synthase antibody3-bisphosphoglycerate mutase antibody3-bisphosphoglycerate synthase antibody3-diphosphoglycerate mutase antibodyAb2 098 antibodyAI323730 antibodyAL022789 antibodyBisphosphoglycerate mutase antibodyBPG dependent PGAM antibodyBPG-dependent PGAM antibodyBPGM antibodyC86192 antibodyDPGM antibodyErythrocyte 2,3 bisphosphoglycerate mutase antibodyerythrocyte antibodyPMGE_HUMAN antibody
see all
Anti-BPGM antibody images
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Western blot - Anti-BPGM antibody (ab112957)Anti-BPGM antibody (ab112957) at 1/100 dilution + MDA-MB-435 cell lysate at 35 µg
Predicted band size : 30 kDa -
Western blot - Anti-BPGM antibody (ab112957)All lanes : Anti-BPGM antibody (ab112957) at 1/100 dilution
Lane 1 : Nontransfected 293 cell lysate
Lane 2 : 293 cell lysate transiently transfected with the BPGM gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 30 kDa
References for Anti-BPGM antibody (ab112957)
ab112957 has not yet been referenced specifically in any publications.
