If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab16781 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
I am studying the chicken meiosis and I would need to know the specific secuence of the synthethic peptide of the Brca1 antibodies (ab16781 and ab16780) to decide the buying of these antibodies. thank you in advance |
|||||||||
ANSWER: |
Thank you for your enquiry. Unfortunately, the only information we have is that the target epitopes are within the N-terminal 304 amino acids of BRCA1. No further characterization was done in-house, and I have not been able to identify citations of more extensive characterization. I am sorry I can't be of more assistance in this occasion. Should you need any further information, please do not hesitate to contact me. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab16781 (1/200) detecting BRCA1 in HeLa cells in conjunction with a goat anti-mouse secondary antibody conjugated to Cy3 (red). Cells were also counterstained with DAPI in order to highlight the nucleus (blue), treated with Bleomycin and incubated with an antibody against Histone H2AX in order to create and expose DNA double strand breaks (green). Please refer to abreviews for further details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
IHC image of ab16781 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab16781, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
2
Call 01223 696 000 or contact us