BRCA1 antibody [MS13] (ab16781)

Product Name 

BRCA1 antibody [MS13]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MS13] to BRCA1

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu

Epitope 

Within the N-terminal 304 amino acids of BRCA1.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
ICC/IF: 1/200 - please refer to abreview for further details.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 207 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

MCF7 cells.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> RING Finger E3 Ligase
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCA1
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation

Relevance 

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MS13

Myeloma 

NS1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

See below for BRCA1 antibody [MS13] images, references, products related to ab16781 and other tools.

BRCA1 antibody [MS13] images:

References for BRCA1 antibody [MS13] (ab16781)

  This clone has been used in:

Scully R et al. Location of BRCA1 in human breast and ovarian cancer cells. Science 272:123-6 (1996). PubMed: 8600523
If you publish research using ab16781 please let us know so that we can cite the reference on this datasheet.

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