Overview

  • Product nameAnti-BRCA1 antibodySee all BRCA1 primary antibodies ...
  • Description
    Rabbit polyclonal to BRCA1
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide (Human) - which represents aportion within the last 100 amino acids of the human Breast Cancer Gene 1 (GenBank PID 627392), conjugated to KLH.

  • Positive control
    • HeLa nuclear or whole cell extract. Any normal human tissue, especially those containing rapidly proliferating cells such as lymphoid germinal centers.

Properties

Applications

Our Abpromise guarantee covers the use of ab9141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
  • Tissue specificityIsoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
    Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
    Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
  • Sequence similaritiesContains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • DomainThe BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
    The RING-type zinc finger domain interacts with BAP1.
  • Post-translational
    modifications
    Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
    Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
  • Cellular localizationCytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Target information above from: UniProt accession P38398 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • BRAC 1 antibody
    • BRCA 1 antibody
    • BRCA1 antibody
    • BRCA1/BRCA2 containing complex subunit 1 antibody
    • BRCA1/BRCA2-containing complex, subunit 1 antibody
    • BRCA1_HUMAN antibody
    • BRCAI antibody
    • BRCC 1 antibody
    • BRCC1 antibody
    • Breast and ovarian cancer susceptibility protein 1 antibody
    • Breast Cancer 1 antibody
    • Breast Cancer 1 Early Onset antibody
    • Breast cancer type 1 susceptibility protein antibody
    • Breast Ovarian Cancer Susceptibility antibody
    • BROVCA1 antibody
    • IRIS antibody
    • Papillary Serous Carcinoma Of The Peritoneum antibody
    • PNCA4 antibody
    • PPP1R53 antibody
    • Protein phosphatase 1 regulatory subunit 53 antibody
    • PSCP antibody
    • RING finger protein 53 antibody
    • RING finger protein 53 antibody
    • RNF53 antibody
    see all

Anti-BRCA1 antibody images



  • Predicted band size : 220 kDa

References for Anti-BRCA1 antibody (ab9141)

This product has been referenced in:
  • Lixia M  et al. Alternative splicing of breast cancer associated gene BRCA1 from breast cancer cell line. J Biochem Mol Biol 40:15-21 (2007). WB ; Human . Read more (PubMed: 17244477) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells and mouse adult testis)
Loading amount 40 µg
Specification HeLa cells and mouse adult testis
Gel Running Conditions Reduced Denaturing (8)
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Submitted Apr 21 2009

Application Immunoprecipitation
Sample Human Cell lysate - nuclear (osteosarcoma (u2os cells))
Total protein in input 40 µg
Specification osteosarcoma (u2os cells)
Immuno-precipitation step Protein A
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Submitted Mar 06 2007

As stated on the datasheet, this antibody (ab9141 -- Rabbit polyclonal to BRCA 1) is expected to detect a band in excess of 220 kDa.

No, we do not have a blot image for this antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"