Anti-BRCA1 antibody (ab9141)

Overview

• Product nameAnti-BRCA1 antibodySee all BRCA1 primary antibodies ...
• Description
  Rabbit polyclonal to BRCA1
• Tested applicationsIP, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide (Human) - which represents aportion within the last 100 amino acids of the human Breast Cancer Gene 1 (GenBank PID 627392), conjugated to KLH.

• Positive controlHeLa nuclear or whole cell extract. Any normal human tissue, especially those containing rapidly proliferating cells such as lymphoid germinal centers.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAntibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Tumor Suppressors

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Ubiquitylation

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • BRCA1

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • RING Finger E3 Ligase

Applications

Target

• FunctionE3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
• Tissue specificityIsoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
• PathwayProtein modification; protein ubiquitination.
• Involvement in diseaseDefects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
  Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
  Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
• Sequence similaritiesContains 2 BRCT domains.
  Contains 1 RING-type zinc finger.
• DomainThe BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
  The RING-type zinc finger domain interacts with BAP1.
• Post-translational
  modificationsPhosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
  Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
• Cellular localizationCytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.

Target information above from: UniProt accession P38398 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 672 Human
  • Omim: 113705 Human
  • SwissProt: P38398 Human
  • Unigene: 194143 Human

• Alternative names
  BRAC 1 antibodyBRCA 1 antibodyBRCA1 antibody

  BRCA1/BRCA2 containing complex subunit 1 antibodyBRCA1/BRCA2-containing complex, subunit 1 antibodyBRCA1_HUMAN antibodyBRCAI antibodyBRCC 1 antibodyBRCC1 antibodyBreast and ovarian cancer susceptibility protein 1 antibodyBreast Cancer 1 antibodyBreast Cancer 1 Early Onset antibodyBreast cancer type 1 susceptibility protein antibodyBreast Ovarian Cancer Susceptibility antibodyBROVCA1 antibodyIRIS antibodyPapillary Serous Carcinoma Of The Peritoneum antibodyPNCA4 antibodyPPP1R53 antibodyProtein phosphatase 1 regulatory subunit 53 antibodyPSCP antibodyRING finger protein 53 antibodyRING finger protein 53 antibodyRNF53 antibody

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Anti-BRCA1 antibody images

• 

  Western blot - Anti-BRCA1 antibody (ab9141)
  
  
  Predicted band size : 220 kDa