Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
|
ab69799 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab27976 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Since the field has tried several antibodies already to use in clean Westerns against mouse Brca2, I was wondering whether you could provide me with an example Western of this antibody against mouse Brca2. Also, if available, I would like to know the exact peptide against which it is raised, or, at least, the homology between mouse-human of the immunogen. |
|||||||||
ANSWER: |
I have just received feedback from the originator of ab27976 and was informed that the western blot was done with a mouse cell line SV-T2 lysate, we do not have an image currently. The homology of the immunogen between human and mouse is ~77%. I hope this information helps, |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab27976 staining BRCA2 in a xenograft MDA-MB-231 of human breast cancer by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% BSA for 30 minutes at 25°C and then incubated with ab27976 at a 1/50 dilution for 16 hours at 25°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.
Image courtesy of an anonymous Abreview.
1
Call 01223 696 000 or contact us