BRCA2 antibody (ab27976)

Product Name 

BRCA2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BRCA2

Immunogen 

Synthetic peptide mapping to the N terminus of human BRCA2.

Reacts with 
(species key)

Hu, Ms

Specificity 

ab27976 recognises BRCA2.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 384 kDa.
Can be blocked with BRCA2 peptide (ab69799).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: mouse cell line SV-T2 lysate

Cellular localization 

Nuclear, extracellular space and secretory granule.

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

Relevance 

BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Thimerosal MSDS

Purification notes 

This antibody is peptide affinity purified

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for BRCA2 antibody (ab27976)

  This product has been used in:

Lee SA et al. Recovery of deficient homologous recombination in Brca2-depleted mouse cells by wild-type Rad51 expression. DNA Repair (Amst) 8:170-81 (2009). PubMed: 18992372

If you publish research using ab27976 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding BRCA2 antibody

Customer FAQs regarding BRCA2 antibody

Protocols for BRCA2 antibody

Price and availability of products related to BRCA2 antibody

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