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Product Name
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BRCA2 antibody
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See all BRCA2 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BRCA2
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Immunogen
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Synthetic peptide: YTAASRNSHNLEFDGSDSSKNDTV conjugated to KLH, corresponding to amino acids 1324-1347 of Human BRCA2.
BLAST 'YTAASRNSHNLEFDGSDSSKNDTV' with  or 
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately ~380 kDa (predicted molecular weight: 384 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa or MCF-7 cells.
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Cellular localization
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Nuclear, extracellular space and secretory granule.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BRCA2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to BRCA2
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BRCA2 antibody - more information
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Customer reviews (feedback) regarding BRCA2 antibody |
Customer FAQs regarding BRCA2 antibody |
Protocols for BRCA2 antibody |
Price and availability of products related to BRCA2 antibody |
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BRCA2 antibody - related products:
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BRCA2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BRCA2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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