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Product Name
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BRCA2 antibody
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See all BRCA2 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to BRCA2
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Immunogen
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Fusion protein, corresponding to amino acids 188-563 of Human BRCA2.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognizes the BRCA2 gene product, a 390-kDa nuclear protein.
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 390 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear, extracellular space and secretory granule.
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Relevance
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BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Myeloma
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NS1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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Phosphate buffered saline
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Purification notes
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1 mg/ml of antibody purified from ascities fluid by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE chromatography. Prepared in 10mM PBS (pH 7.4).
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BRCA2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for BRCA2 antibody (ab97)
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This product has been used in:
Shin S & Verma IM BRCA2 cooperates with histone acetyltransferases in androgen receptor-mediated transcription. Proc Natl Acad Sci U S A 100:7201-6 (2003). PubMed: 12756300
If you publish research using ab97 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BRCA2
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BRCA2 antibody - more information
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Customer reviews (feedback) regarding BRCA2 antibody |
Customer FAQs regarding BRCA2 antibody |
Protocols for BRCA2 antibody |
Price and availability of products related to BRCA2 antibody |
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BRCA2 antibody - related products:
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BRCA2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about BRCA2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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