BRCA2 (phospho T3349) antibody (ab36459)

Product Name 

BRCA2 (phospho T3349) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BRCA2 (phospho T3349)

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 3300 - 3400 of Human BRCA2, phosphorylated at T3349.

(Peptide available as ab36509.)

Predicted to react 
(species key)

Hu
(Due to sequence homology)

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab36509). It gave a negative result in ELISA against the non-modified equivalent peptide (ab36510). This indicates that it is specific for the modified peptide.
See figure below.
This antibody was shown to work in Western blotting (see Abreview).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear, extracellular space and secretory granule.

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

Relevance 

BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.30 - 0.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for BRCA2 (phospho T3349) antibody images, references, products related to ab36459 and other tools.

BRCA2 (phospho T3349) antibody images:

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'

Customer reviews (feedback) regarding BRCA2 (phospho T3349) antibody

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