![Western blot - BRD3 antibody [2088C3a] (ab50818) image](#ab50818_2.jpg)
![Western blot - BRD3 antibody [2088C3a] (ab50818) image](#ab50818wbhela.jpg)
![Western blot - BRD3 antibody [2088C3a] (ab50818) image](#ab50818wb3T3.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-BRD3 antibody [2088C3a]
See all BRD3 products (5) ...
Description
Mouse monoclonal [2088C3a] to BRD3
Tested applications
Flow Cyt, WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment of BRD3 (Human)
Positive control
WB: HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and FC: HeLa cells. IP: RIPA lysate of HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
Clonality
Monoclonal
Clone number
2088C3a
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 0.5 µg/ml
WB: 1/50Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).
IP: Use at 5 µg/mg of lysate.
ICC/IF: 1/10
Target
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Sequence similarities
Contains 2 bromo domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15059
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
see all
Database links
- Entrez Gene: 8019 Human
- GenBank: NP_031397 Human
- Omim: 601541 Human
- SwissProt: Q15059 Human
- Unigene: 522472 Human
- Unigene: 654869 Human
Anti-BRD3 antibody [2088C3a] images:
Western blot - BRD3 antibody [2088C3a] (ab50818)
![Western blot - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818_2.jpg)
Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg
Secondary
Anti Mouse IgG antibody at 1/2500 dilution
Predicted band size : 80 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Western blot - BRD3 antibody [2088C3a] (ab50818)
![Western blot - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818wbhela.jpg)
Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Western blot - BRD3 antibody [2088C3a] (ab50818)
![Western blot - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818wb3T3.jpg)
Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - BRD3 antibody [2088C3a] (ab50818)
![Immunocytochemistry/ Immunofluorescence - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818ICIF.jpg)
Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution )
Immunoprecipitation - BRD3 antibody [2088C3a] (ab50818)
![Immunoprecipitation - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818IP.jpg)
Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa.
Flow Cytometry - BRD3 antibody [2088C3a] (ab50818)
![Flow Cytometry - BRD3 antibody [2088C3a] (ab50818)](/ps/datasheet/Images/50/ab50818/ab50818_1.jpg)
Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 μg)
References for Anti-BRD3 antibody [2088C3a] (ab50818)
ab50818 has not yet been referenced specifically in any publications.
Publishing research using ab50818? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"