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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-BRD3 antibody
See all BRD3 products (5) ...
Description
Rabbit polyclonal to BRD3
Specificity
This antibody detects endogenous levels of total BRD3 protein.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
A synthesized peptide derived from the internal region of human BRD3.
Positive control
Extracts from A549 cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - BRD3 antibody (ab66002)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects bands of approximately 90, 19, 23 and 38 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Ubiquitous.
Involvement in disease
Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
Sequence similarities
Contains 2 bromo domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ15059
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRD 3 antibody
- Brd3 antibody
- BRD3_HUMAN antibody
see all
Database links
- Entrez Gene: 8019 Human
- Entrez Gene: 67382 Mouse
- GenBank: NP_031397 Human
- Omim: 601541 Human
- SwissProt: Q15059 Human
- SwissProt: Q8K2F0 Mouse
- Unigene: 522472 Human
- Unigene: 654869 Human
see all
Anti-BRD3 antibody images:
Western blot - BRD3 antibody (ab66002)
All lanes : Anti-BRD3 antibody (ab66002) at 1/500 dilution
Lane 1 : Extracts from A549 cells at 5-30ug
Lane 2 : Extracts from A549 cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 80 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 19 kDa,23 kDa,38 kDa. We are unsure as to the identity of these extra bands.
References for Anti-BRD3 antibody (ab66002)
ab66002 has not yet been referenced specifically in any publications.
Publishing research using ab66002? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"