Overview

  • Product nameAnti-BRD8 antibodySee all BRD8 primary antibodies ...
  • Description
    Rabbit polyclonal to BRD8
  • Tested applicationsIHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    LCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFSQKHCASQYSE LLETT

    , corresponding to amino acids 25-75 of Human BRD8.

  • EpitopeThe epitope recognized by ab17968 maps to a region between residues 25 and 75 of human Bromodomain.

Applications

Our Abpromise guarantee covers the use of ab17968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Predicted molecular weight: 120 kDa.
IP Use 2-8µg for 108 cells.

Target

  • RelevanceBRD8 may act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. It interacts with MYC and adenovirus E1A protein.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BRD8 antibody
    • Bromodomain containing 8 antibody
    • p120 antibody
    • Skeletal muscle abundant protein 2 antibody
    • Skeletal Muscle Abundant Protein antibody
    • SMAP antibody
    • SMAP2 antibody
    • Thyroid hormone receptor coactivating protein 120kDa antibody
    • Thyroid Hormone Receptor Coactivating Protein antibody
    see all

Anti-BRD8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 with ab17968 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 120 kDa

    Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.

     

    Ab17968 at the concentrations stated, staining human BRD8 in (A) Nuclear extract (NE; 5, 15 and 50ug) from HeLa cells, (B) nuclear extract (1mg) from HeLa cells. Detection was by chemiluminescence with exposure times of less than 1 minute.

References for Anti-BRD8 antibody (ab17968)

This product has been referenced in:
  • Kim J  et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . Read more (PubMed: 20946988) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"