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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Overview
Product name
Anti-BRIT1 antibody
See all BRIT1 products (4) ...
Description
Rabbit polyclonal to BRIT1
Specificity
This antibody detects a band of ~100kD in U2OS and HEK293 cells (Xu et al. 2004). The band is reduced in U2OS cells treated with BRIT1 siRNA (Xu et al. 2004) showing that the antibody is specific for BRIT1.
Tested applications
IHC-P, IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from residues 300 - 400 of Human BRIT1.
Positive control
This antibody gave a positive signal in Hela, Jurkat and HEK293 whole cell lysates and Hela whole cell lysate Bleomycin Treated (40U/ml) and Hydroxyurea Treated (48hr, 1uM)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> BRCT Domain Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-FoFr: 1/300
WB: 1/500 - 1/1000.Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa).Can be blocked with BRIT1 peptide (ab13737).
Target
Function
Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
Tissue specificity
Expressed in fetal brain, liver and kidney.
Involvement in disease
Defects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait.
Sequence similarities
Contains 3 BRCT domains.
Cellular localization
Cytoplasm > cytoskeleton > centrosome.
Target information above from: UniProt accessionQ8NEM0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRCT repeat inhibitor of TERT expression 1 antibody
- BRIT 1 antibody
- FLJ12847 antibody
see all
Database links
- Entrez Gene: 79648 Human
- Entrez Gene: 244329 Mouse
- NCBI: 13375793 Human
- Omim: 607117 Human
- SwissProt: Q8NEM0 Human
- SwissProt: Q7TT79 Mouse
- Unigene: 656769 Human
- Unigene: 708770 Human
see all
Anti-BRIT1 antibody images:
Western blot - BRIT1 antibody (ab2612)
Lanes 1 - 3 : Anti-BRIT1 antibody (ab2612) at 1/250 dilution
Lanes 4 - 6 : Anti-BRIT1 antibody (ab2612) at 1/500 dilution
Lanes 7 - 9 : Anti-BRIT1 antibody (ab2612) at 1/1000 dilution
Lane 1 : 30 ug of LCL1 whole cell lysate.
Lane 2 : 30 ug of LCL2 whole cell lysate.
Lane 3 : 30 ug of LCL3 whole cell lysate.
Lane 4 : 30 ug of LCL1 whole cell lysate.
Lane 5 : 30 ug of LCL2 whole cell lysate.
Lane 6 : 30 ug of LCL3 whole cell lysate.
Lane 7 : 30 ug of LCL1 whole cell lysate.
Lane 8 : 30 ug of LCL2 whole cell lysate.
Lane 9 : 30 ug of LCL3 whole cell lysate.
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 180 kDa,60 kDa (possible cleavage fragment),70 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time : 15 seconds
LCL1, LCL2, and LCL3 are three patient derived fibroblast cell lines.
The middle band is BRIT1 (denoted by arrow) as determined by treatment with siRNA.
Western blot - BRIT1 antibody (ab2612)
All lanes : Anti-BRIT1 antibody (ab2612) at 1/1000 dilution
Lane 1 : GUS (beta glucuronidase) expression negative control.
Lane 2 : In vitro expressed human BRIT1
Secondary
A rabbit polyclonal to Goat IgG H&L (HRP) secondary antibody was used at a 1:5000 dilution.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab2612 was incubated for one hour at room temperature with the membrane in 5% non-fat dry milk plus PBST.
This image was submitted courtesy of I.Gavvovidis, D. Schindler, Institut fuer Humangenetik, Universitaet Wuerzburg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BRIT1 antibody (ab2612)
Image courtesy of Human Protein Atlas
ab2612 staining in human lung, showing staining of the surface epithelial cells (in brown). Paraffin embedded lung tissue was incubated with ab2612 (1:100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab2612 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
.Immunohistochemistry (PFA perfusion fixed frozen sections) - BRIT1 antibody (ab2612)
Immunohistochemistical detection of BRIT1 using antibody (ab2612) on paraformaldehyde perfusion fixed mouse heart tissue sections. Primary antibody diluted @ 1/300 & incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit conjugated to biotin (1/300). The antibody produced a weak cytoplasmic staining in cardiac cells.
Sophie Pezet, CNRS, Paris, France
Western blot - BRIT1 antibody (ab2612)
All lanes : Anti-BRIT1 antibody (ab2612) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with BRIT1 peptide (ab13737) at 1 µg/ml
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with BRIT1 peptide (ab13737) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with BRIT1 peptide (ab13737) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55-60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Western blot - BRIT1 antibody (ab2612)
All lanes : Anti-BRIT1 antibody (ab2612) at 1 µg/ml (BLOCKED IN 5% MILK)
Lane 1 : Hela Whole Cell Lysate - Bleomycin Treated (40U/ml)
Lane 2 : Hela Whole Cell Lysate - Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 25 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : 105 kDa (why is the actual band size different from the predicted?)
Additional bands at : 19 kDa,55 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes
References for Anti-BRIT1 antibody (ab2612)
This product has been referenced in:
- Wood JLet al. MCPH1 functions in an H2AX-dependent but MDC1-independent pathway in response to DNA damage. J Biol Chem 282:35416-23 (2007). WB; Human.Read more (PubMed: 17925396) »
- Xu Xet al. Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1. J Biol Chem : (2004).Read more (PubMed: 15220350) »
See all 2 publications for this product
Publishing research using ab2612? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"