Recombinant Anti-BRN3A antibody [EP1972Y] (ab81213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1972Y] to BRN3A
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-BRN3A antibody [EP1972Y]
See all BRN3A primary antibodies -
Description
Rabbit monoclonal [EP1972Y] to BRN3A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human BRN3A aa 300-400 (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: Human brain lysate; SW480; Mouse cerebrellum; Rat brain; Rat spinal cord. ICC/IF: HeLa cells. Flow Cyt (intra): SH-SY5Y cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1972Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/90. |
|
WB | (1) |
1/2000. Predicted molecular weight: 43 kDa.
For unpurified use at 1/500 - 1/1000. |
Notes |
---|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/90. |
WB
1/2000. Predicted molecular weight: 43 kDa. For unpurified use at 1/500 - 1/1000. |
Target
-
Function
Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
Tissue specificity
Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye. -
Sequence similarities
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Developmental stage
Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5457 Human
- Entrez Gene: 18996 Mouse
- Entrez Gene: 114503 Rat
- Omim: 601632 Human
- SwissProt: Q01851 Human
- SwissProt: P17208 Mouse
- SwissProt: P20266 Rat
- Unigene: 654522 Human
see all -
Alternative names
- Brain specific homeobox/POU domain protein 3A antibody
- Brain-3A antibody
- Brain-specific homeobox/POU domain protein 3A antibody
see all
Images
-
All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (purified)
Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse cerebellum lysates
Lane 3 : Rat brain lysates
Lane 4 : Rat spinal cord lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaBlocking and diluting buffer: 5% NFDM/TBST.
-
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling BRN3A with purified ab81213 at 1/100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) (unpurified)
Lane 1 : LNCaP
Lane 2 : SW480
Lane 3 : PC-3
Lane 4 : Human brain lysate
Predicted band size: 43 kDa
Observed band size: 43 kDa -
All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (unpurified)
Lane 1 : Adult mouse cerebellum lysate
Lane 2 : Adult mouse lower brainstem lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 2 minutes
-
Overlay histogram showing SH-SY5Y cells stained withunpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab81213 has been referenced in 16 publications.
- Lindborg JA et al. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair. Cell Rep 34:108777 (2021). PubMed: 33657370
- Zhang M et al. Brain and Retinal Abnormalities in the 5xFAD Mouse Model of Alzheimer's Disease at Early Stages. Front Neurosci 15:681831 (2021). PubMed: 34366774
- Bataille A et al. In Vitro Differentiation of Human Skin-Derived Cells into Functional Sensory Neurons-Like. Cells 9:N/A (2020). PubMed: 32316463
- Mak HK et al. MicroRNA-19a-PTEN Axis Is Involved in the Developmental Decline of Axon Regenerative Capacity in Retinal Ganglion Cells. Mol Ther Nucleic Acids 21:251-263 (2020). PubMed: 32599451
- Liu L et al. POU4F1 promotes the resistance of melanoma to BRAF inhibitors through MEK/ERK pathway activation and MITF up-regulation. Cell Death Dis 11:451 (2020). PubMed: 32532957