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Western blot - BSND antibody (ab67648)
(enlarge)
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Product Name
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BSND antibody
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See all BSND antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to BSND
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Immunogen
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Full length BSND protein (Human)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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BSND transfected 293T cell lysate
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Cellular localization
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Cell membrane; Multipass membrane protein. Cytoplasm.
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Research areas
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Neuroscience >> Sensory System >> Auditory system
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
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Relevance
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BSND functions as a beta subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter.
Defects in BSND are the cause of Bartter syndrome type 4, also known as infantile Bartter syndrome with sensorineural deafness. Bartter syndrome refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4 is associated with sensorineural deafness.
BSND is expressed primarily in kidney. Also expressed in specific nephron segments and in the stria vascularis of the inner ear.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BSND antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BSND antibody images, references, products related to ab67648 and other tools.
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BSND antibody images:
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 Western blot - BSND antibody (ab67648)
All lanes : BSND antibody (ab67648) at 1/500 dilution
Lane 1 : BSND transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to BSND
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BSND antibody - more information
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Customer reviews (feedback) regarding BSND antibody |
Customer FAQs regarding BSND antibody |
Protocols for BSND antibody |
Price and availability of products related to BSND antibody |
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BSND antibody - related products:
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BSND antibody - other tools:
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Contact Abcam with a Technical Enquiry about BSND antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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