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Products:Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Overview
Product name
Anti-BSND antibody
See all BSND products (2) ...
Description
Mouse polyclonal to BSND
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-320 of Human BSND (NP_476517.1)
Positive control
293 cell lysate Transfected 293T cell lysate Human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Auditory system
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 35 kDa.
IHC-P: Use a concentration of 3 µg/ml
Target
Relevance
BSND functions as a beta subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter. Defects in BSND are the cause of Bartter syndrome type 4, also known as infantile Bartter syndrome with sensorineural deafness. Bartter syndrome refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4 is associated with sensorineural deafness. BSND is expressed primarily in kidney. Also expressed in specific nephron segments and in the stria vascularis of the inner ear.
Cellular localization
Cell membrane; Multipass membrane protein. Cytoplasm.
Alternative names
- BART antibody
- Bartter syndrome infantile with sensorineural deafness antibody
- Bartter syndrome, infantile, with sensorineural deafness (Barttin) antibody
see all
Database links
- Entrez Gene: 7809 Human
- Omim: 606412 Human
- SwissProt: Q8WZ55 Human
Anti-BSND antibody images:
Western blot - BSND antibody (ab88518)
Anti-BSND antibody (ab88518) at 1 µg/ml + 293 cell lysate at 50 µg
Secondary
Goat anti-mouse IgG at 1/5000 dilution
Predicted band size : 35 kDa
Western blot - BSND antibody (ab88518)
All lanes : Anti-BSND antibody (ab88518) at 1 µg/ml
Lane 1 : BSND transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-mouse IgG at 1/5000 dilution
Predicted band size : 35 kDa
Observed band size : 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BSND antibody (ab88518)
Immunoperoxidase staining of human kidney tissue labelled with ab88518 at 3 µg/ml
References for Anti-BSND antibody (ab88518)
ab88518 has not yet been referenced specifically in any publications.
Publishing research using ab88518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"