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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-BST2 antibody
See all BST2 products (4) ...
Description
Mouse polyclonal to BST2
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length human BST2 protein (NP_004326.1).
Positive control
Human spleen tissue lysate, Jurkat cell lysate and BST2 transfected 293T cell lysate Human kidney tissue HeLa cell
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> NFkB pathway
Tags & Cell Markers >> Cell Type Markers >> Other Cell Types
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cardiovascular >> Blood >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 20 kDa.
IHC-P: Use a concentration of 3 µg/ml
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis.
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level).
Sequence similarities
Belongs to the tetherin family.
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading.
Post-translational
modifications
Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein.
Target information above from: UniProt accessionQ10589
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bone marrow stromal antigen 2 antibody
- Bone marrow stromal cell antigen 2 antibody
- Bone marrow stromal cell antigen antibody
see all
Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
Anti-BST2 antibody images:
Western blot - BST2 antibody (ab88523)
Anti-BST2 antibody (ab88523) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size : 20 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Western blot - BST2 antibody (ab88523)
Anti-BST2 antibody (ab88523) at 1 µg/ml + jurkat cell lysate at 50 µg
Predicted band size : 20 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Western blot - BST2 antibody (ab88523)
All lanes : Anti-BST2 antibody (ab88523) at 1 µg/ml
Lane 1 : BST2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size : 20 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BST2 antibody (ab88523)
ab88523, at 3 µg/ml, staining BST2 in formalin fixed, paraffin embedded human kidney tissue sections by Immunoperoxidase/Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - BST2 antibody (ab88523)
ab88523 at 10 µg/ml staining BST2 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
References for Anti-BST2 antibody (ab88523)
ab88523 has not yet been referenced specifically in any publications.
Publishing research using ab88523? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"