Recombinant Anti-BTF antibody [EPR9980(2)] (ab181240)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9980(2)] to BTF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BTF antibody [EPR9980(2)]
See all BTF primary antibodies -
Description
Rabbit monoclonal [EPR9980(2)] to BTF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, RAW64.7, PC-12, Jurkat and MCF-7 whole cell lysates ICC/IF: HepG2 and MCF-7 cells IHC-P: Human colon, gastric cancer and ovarian cancer, mouse testis, and rat liver tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9980(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 106 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 |
|
ICC/IF |
1/1000.
For unpurified use at 1/500 |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 106 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 |
ICC/IF
1/1000. For unpurified use at 1/500 |
Target
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Function
Death-promoting transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9774 Human
- Entrez Gene: 72567 Mouse
- Entrez Gene: 293017 Rat
- Omim: 612588 Human
- SwissProt: Q9NYF8 Human
- SwissProt: Q8K019 Mouse
- Unigene: 486542 Human
- Unigene: 294783 Mouse
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Alternative names
- Bcl 2 associated transcription factor antibody
- Bcl-2-associated transcription factor 1 antibody
- BCL2 associated transcription factor 1 antibody
see all
Images
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All lanes : Anti-BTF antibody [EPR9980(2)] (ab181240) at 1.5 µg/ml (purified)
Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : RAW64.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified ab181240 at 1:1000 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
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Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
All lanes : Anti-BTF antibody [EPR9980(2)] (ab181240) at 1/10000 dilution (Unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat anti-Rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Lanes 2-3 : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181240 has been referenced in 3 publications.
- Xia S et al. Sublethal heat treatment promotes breast cancer metastasis and its molecular mechanism revealed by quantitative proteomic analysis. Aging (Albany NY) 14:1389-1406 (2022). PubMed: 35150481
- Roblain Q et al. Intravitreal injection of anti-miRs against miR-142-3p reduces angiogenesis and microglia activation in a mouse model of laser-induced choroidal neovascularization. Aging (Albany NY) 13:12359-12377 (2021). PubMed: 33952723
- Ma Z et al. Role of BCLAF-1 in PD-L1 stabilization in response to ionizing irradiation. Cancer Sci 112:4064-4074 (2021). PubMed: 34251713