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Western blot - BTK antibody (ab25971)
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Immunocytochemistry - BTK antibody (ab25971)
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Product Name
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BTK antibody
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See all BTK antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to BTK
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Immunogen
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Synthetic peptide, corresponding to 16 N terminal amino acids near the amino terminus of Human BTK.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa. Can be blocked with BTK peptide (ab39736).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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U937 cell lysate.
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Cellular localization
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Cytoplasmic and membrane associated.
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Relevance
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Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/BTK inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal BTK activity.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: PBS. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BTK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BTK antibody images, references, products related to ab25971 and other tools.
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BTK antibody images:
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 Western blot - BTK antibody (ab25971)
Lane 1 : BTK antibody (ab25971) at 0.5 µg/ml
Lane 2 : BTK antibody (ab25971) at 1 µg/ml
Lane 3 : BTK antibody (ab25971) at 2 µg/ml
Lane 1 : for analysis of U937 cell lysate (15ug lysate/lane)
Lane 2 : for analysis of U937 cell lysate (15ug lysate/lane)
Lane 3 : for analysis of U937 cell lysate (15ug lysate/lane)
Predicted band size : 73 kDa
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Immunocytochemistry - BTK antibody (ab25971)
ab52971 at 10µg/ml staining BTK in Daudi cells by ICC/IF
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Search PubMed (MEDLINE) for references to BTK
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BTK antibody - more information
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Customer reviews (feedback) regarding BTK antibody |
Customer FAQs regarding BTK antibody |
Protocols for BTK antibody |
Price and availability of products related to BTK antibody |
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BTK antibody - related products:
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BTK antibody - other tools:
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Contact Abcam with a Technical Enquiry about BTK antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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