Overview

  • Product nameAnti-BTK antibody
    See all BTK primary antibodies
  • Description
    Rabbit polyclonal to BTK
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Ramos cell lysate Human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab71765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • Cellular localizationCytoplasm. Membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Agammaglobulinaemia tyrosine kinase antibody
    • AGMX 1 antibody
    • AGMX1 antibody
    • AT antibody
    • ATK antibody
    • B cell progenitor kinase antibody
    • B-cell progenitor kinase antibody
    • BPK antibody
    • Bruton agammaglobulinemia tyrosine kinase antibody
    • Bruton tyrosine kinase antibody
    • Bruton’s Tyrosine Kinase antibody
    • Btk antibody
    • BTK_HUMAN antibody
    • IMD 1 antibody
    • IMD1 antibody
    • MGC126261 antibody
    • MGC126262 antibody
    • OTTHUMP00000063593 antibody
    • PSCTK 1 antibody
    • PSCTK1 antibody
    • Tyrosine protein kinase BTK antibody
    • Tyrosine-protein kinase BTK antibody
    • XLA antibody
    see all

Anti-BTK antibody images

  • Anti-BTK antibody (ab71765) at 1/100 dilution + Ramos cell lysate at 12.5 µg

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 30 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-BTK antibody (ab71765) at 1/500 dilution

    Lane 1 : Non transfected HEK 293 cell lysate
    Lane 2 : BTK transfected HEK 293 cell lysate

    Lysates/proteins at 1 µg per lane.


    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 105 kDa,130 kDa,35 kDa,40 kDa,48 kDa,51 kDa,55 kDa,97 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab71765 at 1/50 dilution followed by use of a peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-BTK antibody (ab71765)

ab71765 has not yet been referenced specifically in any publications.

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