Anti-BTK antibody (ab71765)

Overview

• Product nameAnti-BTK antibodySee all BTK primary antibodies ...
• Description
  Rabbit polyclonal to BTK
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Positive controlRamos cell lysate Human breast carcinoma tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
• Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
  Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
  Contains 1 Btk-type zinc finger.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• Post-translational
  modificationsAutophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
• Cellular localizationCytoplasm. Membrane. Nucleus.

Target information above from: UniProt accession Q06187 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 695 Human
  • Omim: 300300 Human
  • SwissProt: Q06187 Human
  • Unigene: 159494 Human

• Alternative names
  Agammaglobulinaemia tyrosine kinase antibodyAGMX 1 antibodyAGMX1 antibody

  AT antibodyATK antibodyB cell progenitor kinase antibodyB-cell progenitor kinase antibodyBPK antibodyBruton agammaglobulinemia tyrosine kinase antibodyBruton tyrosine kinase antibodyBruton’s Tyrosine Kinase antibodyBtk antibodyBTK_HUMAN antibodyIMD 1 antibodyIMD1 antibodyMGC126261 antibodyMGC126262 antibodyOTTHUMP00000063593 antibodyPSCTK 1 antibodyPSCTK1 antibodyTyrosine protein kinase BTK antibodyTyrosine-protein kinase BTK antibodyXLA antibody

  see all

Anti-BTK antibody images

• 

  Western blot - BTK antibody (ab71765)
  Anti-BTK antibody (ab71765) at 1/100 dilution + Ramos cell lysate at 12.5 µg
  
  Predicted band size : 76 kDa
  Observed band size : 76 kDa
  Additional bands at : 30 kDa,35 kDa. We are unsure as to the identity of these extra bands.
• 

  Western blot - BTK antibody (ab71765)
  All lanes : Anti-BTK antibody (ab71765) at 1/500 dilution
  
  Lane 1 : Non transfected HEK 293 cell lysate
  Lane 2 : BTK transfected HEK 293 cell lysate
  
  Lysates/proteins at 1 µg per lane.
  
  
  Predicted band size : 76 kDa
  Observed band size : 76 kDa
  Additional bands at : 105 kDa,130 kDa,35 kDa,40 kDa,48 kDa,51 kDa,55 kDa,97 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BTK antibody (ab71765)
  Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab71765 at 1/50 dilution followed by use of a peroxidase-conjugated secondary antibody and DAB staining.