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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Overview
Product name
Anti-BTK (phospho Y550) antibody
See all BTK products (13) ...
Description
Rabbit polyclonal to BTK (phospho Y550)
Specificity
Detects endogenous levels of BTK only when phosphorylated at tyrosine 550.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 550 (D-E-YP-T-S).
Positive control
Extracts from HeLa cells treated with H2O2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Western blot - BTK (phospho Y550) antibody (ab52192)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/40000ELISA: 1/40000
WB: 1/500 - 1/1000.Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
Target
Function
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
Involvement in disease
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Post-translational
modifications
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
Cellular localization
Cytoplasm. Membrane. Nucleus.
Target information above from: UniProt accessionQ06187
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATK antibody
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
see all
Database links
- Entrez Gene: 695 Human
- Entrez Gene: 12229 Mouse
- Omim: 300300 Human
- SwissProt: Q06187 Human
- SwissProt: P35991 Mouse
- Unigene: 159494 Human
- Unigene: 4475 Mouse
Anti-BTK (phospho Y550) antibody images:
Western blot - BTK (phospho Y550) antibody (ab52192)
All lanes : Anti-BTK (phospho Y550) antibody (ab52192) at 1/500 dilution
Lane 1 : extracts from HeLa cells, treated with H2O2
Lane 2 : extracts from HeLa cells, treated with H2O2 with blocking peptide
Predicted band size : 76 kDa
Observed band size : 76 kDa
References for Anti-BTK (phospho Y550) antibody (ab52192)
This product has been referenced in:
- Kubo Tet al. Augmented TLR9-induced Btk activation in PIR-B-deficient B-1 cells provokes excessive autoantibody production and autoimmunity. J Exp Med 206:1971-82 (2009). WB; Mouse.Read more (PubMed: 19687229) »
See 1 publication for this product
Publishing research using ab52192? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"