Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)

Overview

• Product nameAnti-BTK (phospho Y551) antibody [EP267Y]See all BTK primary antibodies ...
• Description
  Rabbit monoclonal [EP267Y] to BTK (phospho Y551)
• SpecificityThe antibody will detect BTK phosphorylation on Tyrosine 551.
• Tested applicationsWB, ICC more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic phospho-peptide corresponding to residues surrounding Tyrosine 551 of human BTK.

• Positive controlK562 cell lysate, Jurkat cell lysate (Pervanadate treated)
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberEP267Y
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
• Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
  Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
  Contains 1 Btk-type zinc finger.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• Post-translational
  modificationsAutophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
• Cellular localizationCytoplasm. Membrane. Nucleus.

Target information above from: UniProt accession Q06187 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 695 Human
  • Omim: 300300 Human
  • SwissProt: Q06187 Human
  • Unigene: 159494 Human

• Alternative names
  Agammaglobulinaemia tyrosine kinase antibodyAGMX 1 antibodyAGMX1 antibody

  AT antibodyATK antibodyATK antibodyB cell progenitor kinase antibodyB-cell progenitor kinase antibodyBPK antibodyBruton agammaglobulinemia tyrosine kinase antibodyBruton tyrosine kinase antibodyBruton’s Tyrosine Kinase antibodyBtk antibodyBTK_HUMAN antibodyIMD 1 antibodyIMD1 antibodyMGC126261 antibodyMGC126262 antibodyOTTHUMP00000063593 antibodyPSCTK 1 antibodyPSCTK1 antibodyTyrosine protein kinase BTK antibodyTyrosine-protein kinase BTK antibodyXLA antibody

  see all

Anti-BTK (phospho Y551) antibody [EP267Y] images

• 

  Western blot - BTK (phospho Y551) antibody [EP267Y] (ab40770)
  All lanes : Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770) at 1/2000 dilution
  
  Lane 1 : K562 cell lysate non-treated
  Lane 2 : K562 cell lysate + Pervanadate
  Lane 3 : Jurkat cell lysate non-treated
  Lane 4 : Jurkat cell lysate + Pervanadate
  
  
  Predicted band size : 74 kDa
  Observed band size : 74 kDa