BTK protein (Tagged) (ab42616)

Product Name 

BTK protein (Tagged)

Product type 

Proteins and Peptides

Peptide 

Full length human BTK with N-terminal proprietary tag.

Specificity 

Specific Activity : >450 U/mg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 0.75ug/ml recombinant BTK.

Tested applications 
(see key)

Inhib

Application notes 
(see key)

Recommended dilutions
Inhib: Use at an assay dependent dilution. This protein is useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other

Relevance 

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation. The PKC/BTK inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal BTK activity.

Storage buffer 

Preservative: None
Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, 10mM reduced Glutathione, pH 8.0

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

Purity: >70% by SDS-PAGE.

Form 

Liquid

Concentration 

Batch dependent within range: 0.36 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This protein was expressed in Baculovirus infected Sf9 cells.

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