Anti-Bacillus anthracis lethal factor antibody [BAL0106] (ab8243)

Inquiry: Stability of EasyLink HRP Conjugation Kit, ab102891, lot GR58311-1, stored at -20C, how long is it stable, i.e., fit for purpose? Stability of ab8243, lot 773780, stored at -20C, how long is it stable, i.e., fit for purpose? Please reply by email, thanks.

ANSWER:

Thank you for contacting us.
When ab8243 is stored properly aliquoted and at -20C, it should be viable for up to 5 years.
When ab102891 is stored properly at -20C, the kit should be viable for a year. Once the labeling is done with the kit, the labeled antibody should be viable for 6 months when stored at 4C.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
http://www.abcam.com/abreviews

Phone looking for a matched pair.

ANSWER:

Thank you for contacting us.

I have had the oportunity to look over the data regarding the antibodies we had talked about this morning. I am confident that your assessment of those products we correct as ab8242 and ab24451 have been created from the same clone with the addition of the HPR on ab24451. As sandwich ELISAs have been tested using ab8243 and ab24451, using ab8243 with ab8242 will should work as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Were these antibodies raised against spores or vegetative cells? What is the recommended dilution for use in ELISA?

ANSWER:

Thank you for your enquiry. Both ab8243 and ab24451 were generated using highly purified lethal factor antigen of Bacillus anthracis. These two antibodies can be used together in a sandwich ELISA where ab8243 is the coating antibody and ab24451 is the conjugate antibody. To use these antibodies in ELISA we would recommend start at a concentration of 5 ug/ml but the end-user should optimize based upon results. Please contact us again if you have any additional questions.

The calibration curve for ELISA on the datasheet states that ab8242 was used for coating, and ab8243 as a HRP conjugate. Is ab8243 HRP conjugated?

ANSWER:

Thank you for your enquiry.

Ab8243 is not HRP conjugated. You may use Ab24451 for detection in place of this antibody, as it is HRP conjugated.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

your B. anthracis antibody #AB8242 should be used as the "conjugate" along with AB8243 as the coating antibody. Does this mean that AB8242 is conjugated (to HRP?)? If it is not conjugated, which secondary antibody is recommended? How can it be avoided that the secondary antibody does not only detect AB8242 but also the coated AB8243 (both are mouse monoclonals)?

ANSWER:

Thank you for your enquiry. Our source has replied: "The normal presentation of the antibodies is non-conjugated, but we are also offering them in conjugated form. We are normally not using anti-mouse IgG secondary antibodies, we are using hrp-conjugated anti-B.anthracis antibodies (HRP-conjugated clone BAL0105, when using the pair BAL0105-BAL0106 in sandwich ELISA)". Would your customer be happy with a vial of BAL0105-HRP conjugated or a refund? Please let me know and I will arrange this accordingly. My apologies for this problem,