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Background Reducing Buffer (ab64234)

Submit an Abreview Q&A (11)

Overview

  • Product nameBackground Reducing Buffer
  • Tested applicationsELISA, IHC-P, WB more details

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.40
    Preservative: 0.5% Sodium azide
    Constituents: Tris buffered saline, 2% BSA, 0.2% Casein
  • Concentration information loading...
  • Research Areas
  • RelevanceBackground reducing buffer has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory.

    • Applications

    Our Abpromise guarantee covers the use of ab64234 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    IHC-P IHC-P: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    References for Background Reducing Buffer (ab64234)

    ab64234 has not yet been referenced specifically in any publications.

    Publishing research using ab64234 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab64234 Submit a Question for ab64234

    Displaying 1 - 10 of 11 results for Abreviews and Q&A

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    Our customer is looking for an antibody diluent for IHC and I searched ab64234 Background Reducing Buffer.
    Is this antibody diluent?



    ab64234 has been optimized to be an effective blocker for IHC. It can also be directly used as primary antibody diluent.

    Dear technical team,

    Our customer would like to purchase several reagents for IHC setting.
    He has only mouse host antibodies now but,there's a possibility to use rabbit host antibody additionally, I recommended ab80436.
    But I'm confus...

    Read More

    Thank you for your enquiry and your interest in our products.

    I understand from your previous e-mail that your customer will perform IHC-P with samples from either rat or rabbit.

    I can confirm that ab80436 EXPOSE Mouse and Rabbit Speci...

    Read More

    Dear technical team,

    Our customer would like to purchase several reagents for IHC setting.
    He has only mouse host antibodies now but,there's a possibility to use rabbit host antibody additionally, I recommended ab80436.
    But I'm confus...

    Read More

    Thank you for your enquiry and interest in our products.

    In order to be able to help your customer further, it would be important and useful for me to know the species and sample type your customer would like to use. Could you please confirm? Read More

    I am currently looking for a kit to help my signal-noise ratio in immunofluorescence, and I came across this product: Background Reducing Buffer (ab64234) On the data sheet, it says "Constituents: Tris buffered saline, 2% BSA, 0.2% Casein." May ...

    Read More

    Thank you for your inquiry.

    Unfortunately, we are not able to release the compounds for this particularproduct as it is proprietary information.

    We aim to provide as much information as possible to our customers, so I am sorry that this ...

    Read More

    The customer kindly called to discuss issues with non-specific banding at around the expected size for VLDL.

    Thank you for contacting us.


    As we had spoken of I have instructed our products team to add the blocking peptide for this ab92943. The product number is ab102057 and can be found at the following link: http://www.abcam.com/VLDL-Receptor-pep...

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    We've now run more extensive tests on the antibody, and have used 'blot certified' BSA as a blocking reagent, but the results are still poor with this antibody. Please could we have a refund.

    Thank you for contacting us. Your credit note ID is. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following wa...

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    Hi, I'll try again all this changes to see if I can improve the quality of the band of Rhodopsin, but what about the size of the band? Is the correct size? And Can you please send me if you have reviews using this ab, Do you have publications with this...

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    Thank you for contacting us.

    The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational mo...

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    I need help again, I send you the file of my WB with the Rhodopsin ab98887 you report the ab is around 39kDa if you see the image of the wb the band maybe I think it is less than 37kDa, what do you think? And I am very surprise how the ab are up the 50 to...

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    Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

    After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark areas...

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    Two different blots attached – really poor! Other primary antibodies used on these same blots worked will with very little problem. I don't have abcam confirmation number you mention, as I never received this, and neither does our purchasing office. Sure...

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    Thank you for sending those images to me. For reference your Abcam order number is . After reviewing you images it appears as the anti-GAPDH antibody is recognizing its target very well. However, there does appear to be a reaction somewhere which is ca...

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    I ordered the anti-TIM1 antibody Ab47635 but I have been having no success with western blots. I am trying to detect KIM-1 (TIM-1) in lysate from whole kidneys. According to many published papers, KIM-1 increases in the kidney following injury. However, w...

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    Thank you for contacting abcam.

    The image on our datasheet for this product ddoes show high non-specific background as well which seemed to be tamed but not eliminated by using a much lower dilution of the product. In that particular case 1ug/m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"