Background Reducing Buffer (ab64234)

Overview

  • Product nameBackground Reducing Buffer
  • Tested applicationsELISA, IHC-P, WBmore details

Properties

  • RelevanceBackground reducing buffer has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory.

Applications

Our Abpromise guarantee covers the use of ab64234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References for Background Reducing Buffer (ab64234)

ab64234 has not yet been referenced specifically in any publications.

Product Wall



ab64234 has been optimized to be an effective blocker for IHC. It can also be directly used as primary antibody diluent.

Thank you for your enquiry and your interest in our products.

I understand from your previous e-mail that your customer will perform IHC-P with samples from either rat or rabbit.

I can confirm that ab80436 EXPOSE Mouse and Rabbit Sp...

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In order to be able to help your customer further, it would be important and useful for me to know the species and sample type your customer would like to use. Could you please confirm?...

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Unfortunately, we are not able to release the compounds for this particularproduct as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that th...

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As we had spoken of I have instructed our products team to add the blocking peptide for this ab92943. The product number is ab102057 and can be found at the following link: http://www.abcam.com/VLDL-Receptor-...

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Thank you for contacting us. Your credit note ID is. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following...

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The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational...

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Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark ar...

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Thank you for sending those images to me. For reference your Abcam order number is . After reviewing you images it appears as the anti-GAPDH antibody is recognizing its target very well. However, there does appear to be a reaction somewhere which is...

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The image on our datasheet for this product ddoes show high non-specific background as well which seemed to be tamed but not eliminated by using a much lower dilution of the product. In that particular case 1u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"