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- Secondary antibodies
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Product name
Anti-Bad antibody [64103]
See all Bad products (27) ...
Description
Rat monoclonal [64103] to Bad
Specificity
This antibody will also detect a 32 kDa band that comigrates with recombinant Bad on immunoblots.
Tested applications
Dot Blotmore details
Cross reactivity
Reacts with
Mouse
Does not react with
Human
Immunogen
Recombinant N-Termanal fragment (Mouse).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
64103
Isotype
IgG2a
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot Blot
Application notes
Dot: a working antibody concentration of approximately 1 µg/ml is recommended using extracts from 2 x 106 mouse cytotoxic T cell line (CTLL2) or L929 fibroblast cells in an enhanced chemiluminescent (ECL) assay.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Tissue specificity
Expressed in a wide variety of tissues.
Sequence similarities
Belongs to the Bcl-2 family.
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Post-translational
modifications
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
Cellular localization
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
Target information above from: UniProt accessionQ92934
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BAD antibody
- BAD_HUMAN antibody
- BBC 2 antibody
see all
Database links
- Entrez Gene: 12015 Mouse
- SwissProt: Q61337 Mouse
- Unigene: 4387 Mouse
References for Anti-Bad antibody [64103] (ab10520)
ab10520 has not yet been referenced specifically in any publications.
Publishing research using ab10520? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"