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Products:Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
Anti-Bad antibody [Y208] (ab32445)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32445? Please let us know so that we can cite the reference in this datasheet
ab32445 has been referenced in 1 publications.
- Maes OC et al. Stepwise up-regulation of microRNA expression levels from replicating to reversible and irreversible growth arrest states in WI-38 human fibroblasts. J Cell Physiol 221:109-19 (2009). PubMed: 19475566
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Bad antibody [Y208] (ab32445)](/ps/datasheet/Images/32/ab32445/ab32445wb.gif)
Western blot - Bad antibody [Y208] (ab32445)
Anti-Bad antibody [Y208] (ab32445) at 1/5000 dilution + Hela cell lysate
Predicted band size : 18 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)](/ps/datasheet/Images/32/ab32445/ab32445ihc.jpg)
Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)
Immunohistochemical staining of paraffin-embedded human lymphoma using ab32445 at 1/100 dilution.
](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-1.jpg)
Immunocytochemistry/ Immunofluorescence-Bad antibody [Y208](ab32445)
ICC/IF image of ab32445 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32445, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-3.jpg)
Flow Cytometry-Bad antibody [Y208](ab32445)
Overlay histogram showing MCF-7 cells stained with ab32445 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32445, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
![Bad antibody [Y208] for WB in Human (32445)](/ps/Reviews/Images/ab8956_43596.jpg)
Bad antibody [Y208] for WB in Human (32445)
![Bad antibody [Y208] for IP in Human (32445)](/ps/Reviews/Images/ab9082_46082.jpg)
Bad antibody [Y208] for IP in Human (32445)
![Anti-Bad antibody [Y208] for Western blot in Human (32445)](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-15.jpg)
Anti-Bad antibody [Y208] for Western blot in Human (32445)
![Bad antibody [Y208] for IHC-P in Human (32445)](/ps/Reviews/Images/ab9003_43610.jpg)
Bad antibody [Y208] for IHC-P in Human (32445)
![Anti-Bad antibody [Y208] for Immunocytochemistry in Mouse (32445)](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-22.JPG)
Anti-Bad antibody [Y208] for Immunocytochemistry in Mouse (32445)
![Bad antibody [Y208] for Flow Cytometry in Human (32445)](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-7.JPG)
Bad antibody [Y208] for Flow Cytometry in Human (32445)
![Anti-Bad antibody [Y208] for Western blot in Human (32445)](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-11.JPG)
Anti-Bad antibody [Y208] for Western blot in Human (32445)
![Bad antibody [Y208] for Immunocytochemistry/ Immunofluorescence in Human (32445)](/ps/datasheet/images/32/ab32445/Bad-Primary-antibodies-ab32445-5.jpg)
Bad antibody [Y208] for Immunocytochemistry/ Immunofluorescence in Human (32445)
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