Anti-Bad antibody [Y208] (ab32445)

Dear Technical service,
Which antibodies could you recommended as a 2nd step to the following products:
ab32445 and ab33862 for flow cytometry?
Awaiting your answer.
Best regards.

ANSWER:

Thank you for contacting us.
There is a wide range of secondaries suitable to detect either ab32445 or ab33862 in Flow Cytometry. I have conducted a search through our Secondaries search tool in the Abcam Website and found more than 100 matches, depending on the conjugation dye.
I would recommend you to have a look at the results and depending on the wavelength you are interested in measuring, choose the option that better matches your requirements.
http://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=app&sv=99&sn=Flow Cyt&l=3&fViewMore=1
If you have any doubt about any of the products identified in the search, you need a proforma, or require further assistance, please do not hesitate to contact me back.

Ordered ab90435 by mistake and would like to return it.

ANSWER:

Thank you for contacting Abcam.

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Good afternoon,

I ordered the rabbit monoclonal antibody BAD several months ago. I now have the question if you could let me know if the antibody binds to (immunohistochemistry) the unphosphorylated or phosphorylated form of BAD?

Thank you for your help!

ANSWER:

Thank you for contacting us.

I can confirm that this antibody does recongize the phosphorylated and unphosphorylated form of BAD. Indeed, there is no phosphorylation site on the immunogen sequences used for this antibody.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.