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Western blot - Bad antibody [Y208] (ab32445)
![Western blot - Bad antibody [Y208] (ab32445)](/ps/datasheet/images/ab32445wb.gif)
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Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)
![Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)](/ps/datasheet/images/ab32445ihc.jpg)
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Immunocytochemistry/ Immunofluorescence-Bad antibody [Y208](ab32445)
](\ps\datasheet\images\32\ab32445\Bad-Primary-antibodies-ab32445-1.jpg)
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Product Name
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Bad antibody [Y208]
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See all Bad antibodies (28)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [Y208] to Bad
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Immunogen
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A synthetic peptide corresponding to residues in the N-term of human Bad.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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The antibody does not cross-react with other Bcl-2 members.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-P, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/50.
ICC/IF: 1/250 - 1/500.
IHC-P: 1/100 - 1/250.
IP: 1/100.
WB: 1/2000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Hela cell lysate, human lymphoma.
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Cellular localization
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Mitochondrion outer membrane. Cytoplasm. Note: Upon phosphorylation, locates to the cytoplasm.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Relevance
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Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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Y208
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bad antibody [Y208] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bad antibody [Y208] images, references, products related to ab32445 and other tools.
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Bad antibody [Y208] images:
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 Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)
Immunohistochemical staining of paraffin-embedded human lymphoma using ab32445 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence-Bad antibody [Y208](ab32445)
ICC/IF image of ab32445 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32445, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to Bad
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Bad antibody [Y208] - more information
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Customer reviews (feedback) regarding Bad antibody [Y208] |
Customer FAQs regarding Bad antibody [Y208] |
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Bad antibody [Y208] - related products:
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Bad antibody [Y208] - other tools:
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Contact Abcam with a Technical Enquiry about Bad antibody [Y208] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Bad antibody [Y208] (ab32445)](/index.html?datasheet=32445#ab32445wb.gif)
![Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)](/index.html?datasheet=32445#ab32445ihc.jpg)
](/index.html?datasheet=32445#Bad-Primary-antibodies-ab32445-1.jpg)
