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Anti-Bad antibody (ab97733)

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Overview

Product name

Anti-Bad antibody
See all Bad products (27) ...

Description

Rabbit polyclonal to Bad

Tested applications

ICC/IF, IHC-Fr, IHC-P, IP, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length Human Bad protein (NP_004313).

Positive control

Spleen, lymphoid, Du145 Human prostate carcinoma cells, many cancer cell lines.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Bcl 2 family
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family

  • Immunocytochemistry/ Immunofluorescence - Bad antibody (ab97733)Immunocytochemistry/ Immunofluorescence - Bad antibody (ab97733) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab97733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

     ICC/IF: 1/500 - 1/2000.

    ICC/IF: 1/500 - 1/2000.

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

  • IHC-P

     IHC-P: 1/1000 - 1/5000.

    IHC-P: 1/1000 - 1/5000.

  • IP

     IP: 1/50 - 1/200.

    IP: 1/50 - 1/200.

  • ShowHide

    WB

     WB: 1/1000 - 1/2000. Pre...Read more →

    WB: 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.

Target

Function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Tissue specificity

Expressed in a wide variety of tissues.

Sequence similarities

Belongs to the Bcl-2 family.

Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Post-translational
modifications

Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

Cellular localization

Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.

Target information above from: UniProt accessionQ92934 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BAD antibody
  • BAD_HUMAN antibody
  • BBC 2 antibody
  • BBC2 antibody
  • BBC6 antibody
  • Bcl 2 Antagonist of Cell Death antibody
  • Bcl 2 Binding Component 6 antibody
  • BCL X / BCL 2 Binding Protein antibody
  • BCL X Binding Protein antibody
  • Bcl XL/Bcl 2 Associated Death Promoter antibody
  • Bcl-2-binding component 6 antibody
  • Bcl-2-like protein 8 antibody
  • Bcl-XL/Bcl-2-associated death promoter antibody
  • BCL2 antagonist of cell death antibody
  • BCL2 antagonist of cell death protein antibody
  • BCL2 associated agonist of cell death antibody
  • Bcl2 Associated Death Promoter antibody
  • BCL2 binding component 6 antibody
  • BCL2 binding protein antibody
  • Bcl2 Like 8 Protein antibody
  • Bcl2-L-8 antibody
  • BCL2L8 antibody
  • Proapoptotic BH3 Only Protein antibody
see all

Database links

Anti-Bad antibody images:

  Immunocytochemistry/ Immunofluorescence - Bad antibody (ab97733)

Immunocytochemistry/ Immunofluorescence - Bad antibody (ab97733)

Immunofluorescence analysis of Bad expression using ab97733 at 1/2000, followed by a FITC-conjugated secondary antibody..
Du145 Human prostate carcinoma cells were cultured without (A) or with (B) 1µM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr.

THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).

References for Anti-Bad antibody (ab97733)

ab97733 has not yet been referenced specifically in any publications.

Publishing research using ab97733? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"