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|Sample type||Average %||Range|
|Cell culture extracts||3µg/ml - 200µg/ml|
BAD (Q92934) is a BH3-only pro-apoptotic member of the Bcl-2 family. BAD competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X (L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways. Bad is localized in mitochondrial outer membrane. Upon phosphorylation, Bad locates to the cytoplasm. Bad is expressed in a wide variety of tissues. Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. BAD is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. The phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X (L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. The phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.
|Components||Identifier||1 x 96 tests|
|ab133984 - 10X BAD pSer75 Detector Antibody||1 x 700µl|
|10X HRP Label||1 x 1ml|
|10X Wash Buffer||8209742||1 x 40ml|
|ab62480 - BAD Microplate (12 x 8 antibody coated wells)||1 x 96 tests|
|Extraction Buffer||1 x 15ml|
|Incubation Buffer||1 x 60ml|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab133984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab133984 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"