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Customer is having problems getting this antibody to work in IP. He is using human cells and at the recommended concentration of 1 - 2 µg/ml. Batch # is 20426. Does the supplier have any protocol specifics for this antibody in IP? What type of cells was it tested with? |
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ANSWER: |
The supplier of this antibody has informed me that ab1435 should be used at a concentration of 10 ug/ml for IP. I apologize for the error on the datasheet and have corrected it. I recommend trying ab1435 at 10 ug/ml and please let me know how this works out for you. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Bad (phospho S112) antibody (ab1435) at 10 µg/ml
Lane 2 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 3 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 4 : COS cell lysate. Anti-BAD antibody used as a loading control.
Lane 5 : COS cell lysate. Anti-BAD antibody used as a loading control.
Performed under reducing conditions.
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Lane 2: No TPA treatment.
Lane 3: 5 minute TPA treatment.
Lane 4: 30 minute TPA treatment.
Lane 5: 60 minute TPA treatment.
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